http://192.168.1.40:8080/xmlui/handle/123456789/1140 2026-05-31T13:43:55Z http://192.168.1.40:8080/xmlui/handle/123456789/1845 Production and characterization of somatic hybrids raised through protoplast fusion between edible mushroom strains Volvariella volvacea and Pleurotus florida Chakraborty, Uttara; Sikdar, Samir Ranjan Twelve somatic hybrid lines were raised through polyethylene glycol-mediated intergeneric protoplast fusion between Volvariella volvacea and Pleurotus florida using a double selection method. Four hybrid lines belonging to two distinct colony morphology types could be maintained in culture. Basidiocarps could be generated from two hybrid lines, one of which showed resemblance to the P. florida parent while the other showed intermediate parental morphology. Hybridity of the fusant lines was established on the basis of colony morphology, mycelial growth rate, hyphal traits, fruit body morphology, isozyme and RAPD analysis. Four isozyme activities were analyzed to provide biochemical criteria for detection of polymorphism among the hybrids and their parents. Using 20 random decamers a total of 203 RAPD bands ranging from 0.09 to 1.6 kb were observed. The UPGMA method of clustering exhibited two major phylogenetic clusters, the first of which comprised of the parents, two fruit body generating hybrid lines and their subsequent fruit body derived lines, while the two non-fruit body generating hybrid lines formed the out group of the first major cluster. Screening of a Pleurotus type hybrid line having high biological efficiency and generation of a temperature tolerant Pleurotus type line through backcross mating between a non-fruit body generating somatic hybrid and V. volvacea parent are the key achievements of this fusion programme. DOI: 10.1007/s11274-007-9630-1 2008-08-01T00:00:00Z http://192.168.1.40:8080/xmlui/handle/123456789/1698 Intergeneric protoplast fusion between Calocybe indica (milky mushroom) and Pleurotus florida aids in the qualitative and quantitative improvement of sporophore of the milky mushroom Chakraborty, Uttara; Sikdar, Samir Ranjan This article primarily focuses on the production of somatic hybrid sporophores through PEG-mediated protoplast fusion between Calocybe indica var. APK2 and Pleurotus florida. To screen the hybrid strains a selection strategy was followed based on the differential tolerance of NaCl level by the two parental genera. Basidiocarps could be successfully generated from eight out of fourteen hybrid lines that were maintained in culture. Hybridity of the fusant lines was established on the basis of their colony morphology, mycelial growth rate and hyphal traits, while the fruit-body-generating lines were demarcated on the basis of nature of sporophores, isozyme and RAPD markers. The degree to which the hybrid population differed among themselves and from their parents was assessed by analysing each of these morphological variables by one-way analysis of variance (ANOVA). RAPD bands of the fusant lines were similar to either parental bands or were new non-parental bands, which classified them into the microgenome and macrogenome insertion types. A dendrogram created with the help of UPGMA method of clustering and Euclidean distance exhibited three major clusters, in which the Pleurotus-Calocybe hybrids showed intra-cluster variations. Notably, P. florida was genetically distant from the hybrid lines, while C. indica was phylogenetically the dominant parent. Significant increase in bio-efficiency and gamma-linoleic acid content in these hybrid lines indicated quantitative as well as qualitative improvement of the newly developed somatic hybrids. DOI: 10.1007/s11274-009-0162-8 2010-02-01T00:00:00Z http://192.168.1.40:8080/xmlui/handle/123456789/1420 A (1→6)-β-glucan from a somatic hybrid of Pleurotus florida and Volvariella volvacea: isolation, characterization, and study of immunoenhancing properties Das, Debsankar; Mondal, Subhas; Roy, Sadhan K.; Maiti, Debabrata; Bhunia, Bibhas; Maiti, Tapas K.; Sikdar, Samir Ranjan; Islam, Syed S. A water-soluble immunoenhancing polysaccharide was isolated from the aqueous extract of fruit bodies of somatic hybrid (Pflo Vv5 FB), obtained through protoplast fusion between Pleurotus florida and Volvariella volvacea strains. On the basis of acid hydrolysis, the polysaccharide was found to contain glucose only. Methylation analysis, periodate oxidation along with 1H, DEPT-135, and 13C NMR spectroscopy, including two-dimensional TOCSY, DQF-COSY, NOESY, ROESY, 1H,13C-HMQC, and HMBC experiments showed that the polysaccharide was a (1→6)-β-d-glucan, which was not a constituent of any of the parent mushrooms previously reported. DOI: 10.1016/j.carres.2010.02.028 2010-05-07T00:00:00Z http://192.168.1.40:8080/xmlui/handle/123456789/1389 Structural characterization and study of immunoenhancing and antioxidant property of a novel polysaccharide isolated from the aqueous extract of a somatic hybrid mushroom of Pleurotus florida and Calocybe indica variety APK2 Maity, Kousik; Kar (Mandal), Eshita; Maity, Saikat; Gantait, Sanjoy K; Das, Debsankar; Maiti, Swatilekha; Maiti, Tapas K; Sikdar, Samir Ranjan; Islam, Syed S. A water-soluble polysaccharide was isolated from the aqueous extract of the fruit bodies of somatic hybrid PCH9FB, obtained through intergeneric protoplast fusion between the strains Pleurotas florida and Calocybe indica var. On the basis of total acid hydrolysis, the polysaccharide was found to contain galactose, fucose, and glucose in a molar ratio of nearly 2:1:2. Methylabon analysis and NMR experiments (H-1, C-13, DEPT-135, DQF-COSY, TOCSY, NOESY, ROESY, HMQC, and HMBC) showed that the structure of the repeating unit present in the polysaccharide was D B-D-Glcp 1 down arrow -> 6)-alpha-D-Galpp(1 -> 6)-alpha-D-Galp-(1 -> 6)-alpha-D-Glcp-(1 -> 2 up arrow 1 alpha-L-Fucp This molecule showed macrophage, splenocyte, thymocyte activation as well as antioxidant property. DOI : 10.1016/j.ijbiomac.2010.12.003 2011-03-01T00:00:00Z