http://192.168.1.40:8080/xmlui/handle/123456789/1431 2026-05-31T13:43:56Z http://192.168.1.40:8080/xmlui/handle/123456789/1839 A Microsatellite DNA Marker Developed for Identifying Disease-resistant Population of Giant Black Tiger Shrimp, Penaeus monodon Mukherjee, Kuntal; Mandal, Nripendranath White spot disease caused by white spot syndrome virus (WSSV) poses major problems that result in huge economic losses each year in shrimp aquaculture throughout the world. In the present study, microsatellite-based DNA fingerprints have been compared between naturally occurring WSSV disease-resistant and susceptible populations of giant black tiger shrimp, Penaeus monodon, to find DNA markers. For the first time, we report here a microsatellite locus, which, after amplification by polymerase chain reaction, provides a highly statistically significant DNA fingerprint of 71 bp, only in disease susceptible populations but not in disease-resistant shrimp populations, whereas a 317 bp band is common in both. The absence of the former DNA marker will be very useful to identify disease-resistant broodstock of P. monodon for marker-assisted selection in breeding programs to generate disease-free shrimps (P. monodon) in the aquaculture industry. DOI: 10.1111/j.1749-7345.2009.00250.x 2009-04-01T00:00:00Z http://192.168.1.40:8080/xmlui/handle/123456789/1834 Antioxidant and free radical scavenging activity of Spondias pinnata Hazra, Bibhabasu; Biswas, Santanu; Mandal, Nripendranath Background: Many diseases are associated with oxidative stress caused by free radicals. Current research is directed towards finding naturally-occurring antioxidants of plant origin. The aim of the present study was to evaluate the in vitro antioxidant activities of Spondias pinnata stem bark extract. Methods: A 70% methanol extract of Spondias pinnata stem bark was studied in vitro for total antioxidant activity, for scavenging of hydroxyl radicals, superoxide anions, nitric oxide, hydrogen peroxide, peroxynitrite, singlet oxygen and hypochlorous acid, and for iron chelating capacity, reducing power, and phenolic and flavonoid contents. Results: The extract showed total antioxidant activity with a trolox equivalent antioxidant concentration (TEAC) value of 0.78 +/- 0.02. The IC(50) values for scavenging of free radicals were 112.18 +/- 3.27 mu/ml, 13.46 +/- 0.66 mu g/ml and 24.48 +/- 2.31 mu g/ml for hydroxyl, superoxide and nitric oxide, respectively. The IC(50) for hydrogen peroxide scavenging was 44.74 +/- 25.61 mg/ml. For the peroxynitrite, singlet oxygen and hypochlorous acid scavenging activities the IC(50) values were 716.32 +/- 32.25 mu g/ml, 58.07 +/- 5.36 mu g/ml and 127.99 +/- 6.26 mu g/ml, respectively. The extract was found to be a potent iron chelator with IC(50) = 66.54 +/- 0.84 mu g/ml. The reducing power was increased with increasing amounts of extract. The plant extract (100 mg) yielded 91.47 +/- 0.004 mg/ml gallic acid-equivalent phenolic content and 350.5 +/- 0.004 mg/ml quercetin-equivalent flavonoid content. Conclusion: The present study provides evidence that a 70% methanol extract of Spondias pinnata stem bark is a potential source of natural antioxidants. DOI: 10.1186/1472-6882-8-63 2008-12-09T00:00:00Z http://192.168.1.40:8080/xmlui/handle/123456789/1831 Association of low-density lipoprotein particle size and ratio of different lipoproteins and apolipoproteins with coronary heart disease Biswas, Santanu; Ghoshal, Pradip K; Mandal, Sankar C; Mandal, Nripendranath Background: Worldwide coronary heart disease (CHD) is estimated to be the leading cause of death. Current knowledge about prevention of CHD is mainly derived from developed countries. Therefore, this study aimed to find out the association of CHD with ratios of different lipoproteins and apolipoproteins, LDL particle size, as well as different traditional risk factors in Asian Indian population in Eastern part of India. Methods: Case-control study of 100 patients with CHD and 98 healthy controls were age and sex matched. After clinical evaluation, blood samples were collected for biochemical assays. Results: Multivariate logistic regression analysis found apoB (OR 2.96; 95% CI 1.02-8.54), apoB/HDL-c (OR 4.14; 95% CI 1.33-12.83), nonHDL-c (OR 5.41; 95% CI 2.08-14.10), apoB/apoAI (OR 6.64; 95% CI 2.37-18.57), and LDL particle size (9.59; 95% CI 2.92-31.54) were independently associated with CHD. Area under the ROC curves derived from the model (AUROC 0.947; 95% CI 0.916-0.977) are significantly higher than any other variables. Conclusions: Findings from the multivariate analysis, apoB, apoB/HDL-c, nonHDL-c, apoB/apoAI, and LDL particle size are potent indicators and useful for diagnosis of predisposed CHD. (C) 2008 Japanese College of Cardiology 10.1016/j.jjcc.2008.06.005 2008-10-01T00:00:00Z http://192.168.1.40:8080/xmlui/handle/123456789/1581 Assessment of in vitro antioxidant and free radical scavenging activity of Cajanus cajan Sarkar, Rhitajit; Hazra, Bibhabasu; Mandal, Sourav; Biswas, Santanu; Mandal, Nripendranath The present study was undertaken to evaluate the antioxidant and free radical scavenging activities of 70% methanolic extract of Cajanus cajan leaves using various in vitro assays. The TEAC value 0.84 ± 0.05 for the extract indicates it is an antioxidant. The extract had shown its scavenging activity for different free radicals and 175.73 ± 7.50 μg/ml, 27.17 ± 0.9 μg/ml, 60.78 ± 5.15 μg/ml and 250.29 ± 5.70 μg/ml were determined as the IC50 values for hydroxyl, superoxide, nitric oxide and singlet oxygen radicals, respectively. The sample showed reasonable chelation of Fe2+ ion and inhibition of lipid peroxidation. The phenolic and flavonoid contents of the extract were also determined to be 51.33 ± 0.002 mg/ml gallic acid equivalent and 143.0 ± 0.003 mg/ml quercetin equivalent, respectively. The results showed that 70% methanol extract of C. cajan leaves possesses antioxidant and free radical scavenging properties DOI : 10.2202/1553-3840.1248 2009-09-01T00:00:00Z