http://192.168.1.40:8080/xmlui/handle/123456789/1989 2026-05-31T13:44:02Z http://192.168.1.40:8080/xmlui/handle/123456789/2415 Anther culture and plant regeneration in sugarcane (Saccharum officinarum) Ruhul Baksha, A.K.M.; Alam, R.; Karim, Z.; Mannan, S.K.; Mafizur Rahman, A.B.M.; Gupta, Shrish Chandra Anthers of Saccharum officinarum clone I 273-91 with microspores at mid uninucleate stage, responded to callusing within 60 days after plating on MS medium supplemented with sucrose (3%), 2, 4-D (2 mg/l) and coconut milk (10%). High sucrose concentration in callusing medium was found to be detrimental for callus induction. Frequency of callusing was 12.2% and 18.5% respectively when the anthers were incubated in callus induction medium (MS + 2.0 mg/l NAA or 2, 4-D + 10% coconut milk + 3% Sucrose). Thirty per cent of the androgenic calli differentiated into green plants within 15 days after transfer to regeneration medium (MS+NAA 0.5 mg/l+BA 1.0 mg/l+3% sucrose). Both albino and green plants were obtained from the androgenic calli in regeneration medium and differentiation of green plants were always more with 2, 4-D induced calli in comparison to NAA induced calli. Growth and multiplication of the regenerated shoots was obtained by transferring of individual shoots into multiplication medium (MS liquid medium + 1.0 mg/l NAA + 2.0 mg/l BA), which enabled them to multiply into 10-15 useable shoots within 30 days. Half MS salt solution supplemented with either NAA or IBA (5.0mg/l) was found suitable for rooting and further growth of the shoots. Regenerated androgenic plants showed narrow range of variability for different characters. 2003-09-01T00:00:00Z http://192.168.1.40:8080/xmlui/handle/123456789/2414 In vitro plant regeneration from leaf sheath cultures of sugarcane via organogenesis Alam, R.; Mafizur Rahman, A.B.M.; Gupta, Shrish Chandra vitro morphogenesis was obtained from leaf sheath derived calli of three local varieties (lsd - 16, lsd -28 and Amrita) of sugarcane Saccharum officinarum L). The calli induced in 2,4 - D (0.5mg/l) containing MS medium showed better regenerating capacity than NAA containing one. Genotype specific requirement of auxin for regeneration of shoots was observed in the three varietes of sugarcane. Shoot differentiation started from the first passage of subculture in regeneration medium, reached its peak at third passage but declined beyond fifth passage of subculture. Rooting was induced in MS medium supplemented with high concentration of auxin (5.0 mg/I) and sucrose (5%). Mass propagation of plantlets was attained through in vitro tillering in half strength of liquid MS medium. 2002-09-01T00:00:00Z http://192.168.1.40:8080/xmlui/handle/123456789/2005 Active cellular preform derived from stems of jute and sticks of cane and their suitability for bulk porous Si/SiC ceramics Mandal, Pijush Kanti; Majumdar, Rabindranath; Mukherjee, Kalyan Kumar; Chakrabarti, Omprakash; Maiti, Himadri Sekhar Stems of Jute (Corchorus capsularis L.) and sticks of Cane (Calamus rotang L.), plants of immense economic importance in the Indian subcontinent, were converted into carbonaceous perform (C-preform) maintaining the circular cylindrical shapes in lengths of 0.02-0.05 m by controlled thermal processing. Plant material precursors were characterized by analysis of elemental (C, H, N) and molecular (cellulose, hemicellulose, lignin) compositions, by determination of Bulk Density (BD) and ash content and by optical microscopy and X-ray diffractometry (XRD). C-preforms were also characterized by measurement of BD and by Scanning Electron Microscopy (SEM) and XRD. The C-preforms were further subjected to infiltration with Si-melt (1823-1923 K) under vacuum. Spontaneous infiltration and reaction yielded composite ceramics preserving the morphology of native Jute Stem (JS) and Cane Stick (CS) precursors on macro and micro scale. The materials were found to be duplex composites with Si and beta-SiC as crystalline phases. The end ceramics were characterized by measurement of BD, and also by SEM and by XRD. Measured mean BD of the Si/SiC composites derived from JS and CS were 2190 Kg m(-3) and 2250 Kg m(-3). The respective volume fractions of large diameter (> 100 mu m) bulk pores were 0.134 and 0.204, in the composites derived from JS and CS. Taking into account the measured volume fraction internal pores of 0.11 and 0.149, the volume fractions of SiC were calculated to be 0.136 and 0.307 in the composites derived from JS and CS respectively, closely tallying with those calculated from the C-preform bulk densities. The cellular Si/SiC ceramics derived from JS and CS having special morphologies with long and large porous channels and oriented growth of constituent phases are likely to be suitable for devices such as high temperature insulators, catalyst support structures for gas phase reactions at elevated temperatures, molten metal filters and others. DOI: 10.1007/s10934-007-9180-9 2009-04-01T00:00:00Z