http://192.168.1.40:8080/xmlui/handle/123456789/1134 Sun, 31 May 2026 14:24:27 GMT 2026-05-31T14:24:27Z http://192.168.1.40:80/xmlui/bitstream/id/35c1dde6-ef85-4372-a94c-189fb1786d11/ssm.jpg http://192.168.1.40:8080/xmlui/handle/123456789/1134 http://192.168.1.40:8080/xmlui/handle/123456789/2350 Molecular profiling and development of DNA marker associated with drought tolerance in tea clones growing in Darjeeling Mishra, R. K.; Sen-Mandi, Swati Amplified Fragment Length Polymorphism (AFLP) fingerprints were developed for 29 Darjeeling-grown tea clones. AFLP diversity estimates based on Jaccard's coefficient allowed separation of the 29 clones into three clusters. Genetic relatedness between the clones was found to be at 70% level. Random Amplified Polymorphic DNA (RAPD) analysis of DNA of ten short-listed (on the basis of field performance for drought tolerance) clones using 11 primers, revealed 180 PCR products of which 131 were polymorphic bands. Activity of drought-specific superoxide dismutase (SOD) and ascorbate peroxidase (APX) isozymes was found to be appreciably high in RR17/144, CP1, TV26 and AV2. Regression analysis using peak areas (from scans of stained activity-gel preparation) of Cu-Zn SOD and APX II as dependent variables and RAPD band scores as independent variable revealed that OPAH02 primed DNA band at 1400 by was associated with high activity of the drought tolerance-specific isozymes. Using Fisher's exact test (F-test), this association was found to be at 99.9% confidence level. Sat, 10 Jul 2004 00:00:00 GMT http://192.168.1.40:8080/xmlui/handle/123456789/2350 2004-07-10T00:00:00Z http://192.168.1.40:8080/xmlui/handle/123456789/1662 Seed vigour-related DNA marker in rice shows homology with acetyl CoA carboxylase gene Talai, Subhra; Sen-Mandi, Swati Germination potential, i.e. vigour manifested at sensu stricto germination, was studied in 22 rice varieties to distinguish between the genetic control for low vigour (lv) at fresh harvest and lv after ageing of seeds. For identifying seed vigour-associated DNA marker(s), correlative studies between (1) physiological parameters viz. germination of (a) unaged seeds under optimum and cold stressed condition, (b) aged seeds under optimal condition and (2) growth-associated biochemical parameters viz. alcohol dehydrogenase (ADH) and esterase (EST) activity during early germination of fresh seeds and antioxidant potential in dry embryos of fresh and aged (1 year) seeds were undertaken. ADH and EST activity as well as antioxidant potential showed positive correlation with speed of embryo emergence and early seedling growth of seeds. The reduction of antioxidant potential in aged seeds also appeared proportional to germination performance. Random amplified polymorphic DNA analysis between the varieties was correlated with the above-mentioned vigour parameters, using multiple regression analysis. This led to the identification of four bands associated with the vigour trait at 99% significance level; of these, the similar to 900-bp DNA fragment (named as HVAC 19), through basic local alignment search tool nucleotides analysis, revealed a sequence homology with acetyl CoA carboxylase (ACCase) gene of wheat at 94% similarity level. DOI: 10.1007/s11738-009-0392-2 Fri, 01 Jan 2010 00:00:00 GMT http://192.168.1.40:8080/xmlui/handle/123456789/1662 2010-01-01T00:00:00Z http://192.168.1.40:8080/xmlui/handle/123456789/1463 DNA profiling of Capsicum landraces of Manipur Sanatombi, K.; Sen-Mandi, Swati; Sharma, G. J. Seven chilli landraces of Manipur belonging to three cultivated species of Capsicum (Capsicum annum, Capsicum frutescens, and Capsicum chinense) form economically important food crops of the region. The genotypes were characterized using ten random amplified polymorphic DNA (RAPD) markers. The cluster analysis based on Jaccard's similarity coefficient calculated by UPGMA method differentiated the genotypes into two main cluster groups. One cluster represented the C. annuum genotypes while the other cluster represented the C. frutescens and the C. chinense genotypes. C. chinense genotypes were more close to C. frutescens genotypes. Genetic variation between the C. frutescens genotypes was more than among the C. annuum genotypes and the C. chinense genotypes were the least similar ones. DOI: 10.1016/j.scienta.2010.01.006 Mon, 05 Apr 2010 00:00:00 GMT http://192.168.1.40:8080/xmlui/handle/123456789/1463 2010-04-05T00:00:00Z http://192.168.1.40:8080/xmlui/handle/123456789/1375 Species-specific AFLP markers for identification of Zingiber officinale, Z. montanum and Z. zerumbet (Zingiberaceae) Ghosh, S.; Majumder, P. B.; Sen-Mandi, Swati The Zingiber genus, which includes the herbs known as gingers, commonly used in cooking, is well known for its medicinal properties, as described in the Indian pharmacopoeia. Different members of this genus, although somewhat similar in morphology, differ widely in their pharmacological and therapeutic properties. The most important species of this genus, with maximal therapeutic properties, is Zingiber officinale (garden ginger), which is often adulterated with other less-potent Zingiber sp. There is an existing demand in the herbal drug industry for an authentication system for the Zingiber sp in order to facilitate their commercial use as genuine phytoceuticals. To this end, we used amplified fragment length polymorphism (AFLP) to produce DNA fingerprints for three Zingiber species. Sixteen collections (six of Z. officinale, five of Z. montanum, and five of Z. zerumbet) were used in the study. Seven selective primer pairs were found to be useful for all the accessions. A total of 837 fragments were produced by these primer pairs. Species-specific markers were identified for all three Zingiber species (91 for Z. officinale, 82 for Z. montanum, and 55 for Z. zerumbet). The dendogram analysis generated from AFLP patterns showed that Z. montanum and Z. zerumbet are phylogenetically closer to each other than to Z. officinale. The AFLP fingerprints of the Zingiber species could be used to authenticate Zingiber sp-derived drugs and to resolve adulteration-related problems faced by the commercial users of these herbs. DOI : 10.4238/vol10-1gmr1154 Sat, 01 Jan 2011 00:00:00 GMT http://192.168.1.40:8080/xmlui/handle/123456789/1375 2011-01-01T00:00:00Z