http://192.168.1.40:8080/xmlui/handle/123456789/1136 Sun, 31 May 2026 13:43:06 GMT 2026-05-31T13:43:06Z http://192.168.1.40:80/xmlui/bitstream/id/f511bff4-518f-4b3c-b5cc-da67b472ce5f/PKS.jpg http://192.168.1.40:8080/xmlui/handle/123456789/1136 http://192.168.1.40:8080/xmlui/handle/123456789/2165 Ultrastuctural dimorphism of micropyle determines differential germinability of Sesbania cannabina seeds Das, B.; Saha, Prabir Kumar S. cannabina seeds, obtained from a naturally occurring single population contained both dormant and non-dormant seeds. The water uptake pattern indicated that non-dormant seeds absorbed sufficient water to allow them to germinate whereas dormant seeds failed to germinate due to restricted water entry. Using a scanning electron microscopy testa ultrastructural studies revealed that the closed micropyle and limited opening of the hilar groove restricts water from entering dormant seeds whereas an open type micropyle allows large amounts water uptake and successful germination in non-dormant seeds. So, variability with respect to germinability and testa ultrastructure exists within a single seed lot of S. cannabina. In this case micropyle acts as the determining factor for dimorphism and influences the germinability. Sun, 01 Jan 2006 00:00:00 GMT http://192.168.1.40:8080/xmlui/handle/123456789/2165 2006-01-01T00:00:00Z http://192.168.1.40:8080/xmlui/handle/123456789/2162 Isolation of low erucic acid-containing genotype of Indian mustard (Brassica juncea Czern. and Coss.) through F-1 hybrid anther culture Roy, Amitava; Saha, Prabir Kumar Reciprocal crosses were done between two cultivars; cv. RJ15 and cv. RLM198 of Indian mustard ( Brassica juncea). Anther derived lines designated as A(1) plants, were raised through anther culture from these F-1 hybrid plants. 45% germination was obtained from distinctly shriveled and small A(1) seeds and grown along with the F-2 plants in the same agro- climatic conditions. Subsequently the lines were compared for inheritance pattern between the lines. A normal frequency distribution curve for siliqua per plant was obtained in all the lines reflecting a similar pattern of recombination. Few seeds from the plants of each lines exhibiting high number of siliqua per plant, were isolated for analysis of erucic acid. Three plants in which erucic acid content was lower than the parent cultivars of A(2) generation were identified. This showed that contrasting characters could be obtained from A2 plants where the traits are oligo or monogenic through anther culture. Thu, 16 Nov 2006 00:00:00 GMT http://192.168.1.40:8080/xmlui/handle/123456789/2162 2006-11-16T00:00:00Z http://192.168.1.40:8080/xmlui/handle/123456789/1974 HARDENING - AN ESSENTIAL STEP FOR TISSUE CULTURERAISED PLANTS Roy, Amitava; Saha, Prabir Kumar High humidity is required for acclimatizing tissue culture raised plants after taking them out from rooting media and transferring to pots. In greenhouse, controlling the transpiration of these plants gradually does help them to get adapted in the in vivo climate. Stepwise transition from test tube to greenhouse can reduce loss of plant material. But these steps differ according to plant species in culture. Several techniques, rooting substrate and microclimate(s), are used to harden these plants. The sequentially standardized steps for routine transplantation of tissue culture raised banana plantlets are discussed here. Tue, 01 Jan 2008 00:00:00 GMT http://192.168.1.40:8080/xmlui/handle/123456789/1974 2008-01-01T00:00:00Z