http://192.168.1.40:8080/xmlui/handle/123456789/1138 Sun, 31 May 2026 14:24:29 GMT 2026-05-31T14:24:29Z http://192.168.1.40:80/xmlui/bitstream/id/3dba05f5-0c2b-4424-84d7-07251faae57c/SBP1.jpg http://192.168.1.40:8080/xmlui/handle/123456789/1138 http://192.168.1.40:8080/xmlui/handle/123456789/2346 Development of a potent in vitro source of Phyllanthus amarus roots with pronounced activity against surface antigen of the hepatitis B virus Bhattacharyya, R.; Bhattacharya, Sabita In vitro culture of hairy roots of Phyllanthus amarus induced by Agrobacterium rhizogenes was established. Their growth and ability for in vitro inactivation of hepatitis B virus surface antigen was studied and compared with adventitious roots grown in vitro. The selected hairy root clone HR-1 was capable of growing at a very fast rate, and an approximately 900-fold increase in weight of root biomass was achieved after 4 wk of culture in hormone-free quarter-strength liquid Murashige and Skoog medium with continuous agitation. Non-transformed roots cultured in the presence of 1.0 mg l(-1) (5.71 muM) indole-3-acetic acid increased by 330-fold. The immuno-inactive property of roots was maximal in the crude extract. The hairy roots were shown to possess 85% inhibition (in contrast to 15% in the control) in binding of hepatitis B surface antigen (HBsAg) to its antibody (anti-HBs) after 24 h of incubation with HbsAg-positive sera in vitro at 37degreesC. Out of three fractions selected on the basis of molecular weight components of the extract, the Fraction III containing comparatively lower molecular weight substances (less than or equal to3500) yielded the highest activity. The extract from non-transformed roots was found to possess similar efficiency (87% inhibition). The levels of activity in both types of in vitro-raised roots were higher than those of naturally occurring roots and leafy shoots. The ability of P. amarus hairy root cultures to yield high biomass with the anti-viral property at high levels may provide an alternative source of raw material for more detailed study in the field of pharmaceutical research. DOI: 10.1079/IVP2004560 Wed, 01 Sep 2004 00:00:00 GMT http://192.168.1.40:8080/xmlui/handle/123456789/2346 2004-09-01T00:00:00Z http://192.168.1.40:8080/xmlui/handle/123456789/2170 Conservation and documentation of the medicinal plant resources of India Bhattacharyya, Rajasri; Bhattacharya, Sabita; Chaudhuri, Siddhartha In India, activities in the field of medicinal plants, including conservation of germplasm, have been enhanced significantly during the past couple of decades and a huge volume of data is being generated out of these works. For maintaining the records in a consolidated form, documentation is required to store and manage all information on the related studies. In accordance with the implementation of various plans and programmes, some pioneer organisations started developing databases on medicinal plants. Based on the knowledge on contemporary works, as collected from published literature and websites, this article presents information on current activities in India in two important aspects of the field, namely, (1) conservation of medicinal plants; and (2) management of data generated from such studies. Another important aspect of the article is the announcement of a plant conservation related software, 'PlantCon'. This digitised database contains data of 40 selected nationally prioritised medicinal plants (list enclosed). The notable difference of 'PlantCon' from other databases lies in its conservation-related information which is up-to-date and covers a wide area of Indian geographical sites. The database provides information in a user-friendly manner. DOI: 10.1007/s10531-005-6974-4 Sat, 01 Jul 2006 00:00:00 GMT http://192.168.1.40:8080/xmlui/handle/123456789/2170 2006-07-01T00:00:00Z http://192.168.1.40:8080/xmlui/handle/123456789/2098 In vitro conservation of Plumbago indica - A rare medicinal plant Bhattacharyya, R.; Ray, A.; Gangopadhyay, M.; Bhattacharya, Sabita Present work has been conducted on in vitro conservation of a rare medicinal plant, Plumbago indica, through storage of germplasms derived from micropropagated progenies of the species. Direct regeneration of axillary shoots and their multiplication have been optimized using BAP at a concentration of 2.0 mg/l in MS with 3% sucrose. Plantlets with profusely growing roots, induced by 1.0 mg/l putrescine have been transferred successfully to natural environment as evident from their high survival rate. In vitro storage of P. indica has been developed by (1) reduced growth culture of shoot apex explants for prolonged period without periodic transfer and (2) by preserving encapsulated in vitro generated propagules (artificial seed) for a short term period. Combined application of mannitol (3%) and BAP (2.0 mg/l) resulted in significant reduction in growth rate. After 12 months of storage, cultures survived with 100% success by re-growing in regeneration medium. The alginate-coated beads, made by encapsulating small groups of propagules in each are excellent storable germplasm units, which on germination gave rise to 4-6 plantlets / bead on average on or before 3 months storage at tissue culture room temperature (22°C - 24°C) in absence of light and showed high survival ability. The report on in vitro conservation of this important medicinal plant germplasm may open up new possibilities in this area. Thu, 01 Mar 2007 00:00:00 GMT http://192.168.1.40:8080/xmlui/handle/123456789/2098 2007-03-01T00:00:00Z http://192.168.1.40:8080/xmlui/handle/123456789/1983 An improved micropropagation of Eclipta alba by in vitro priming with chlorocholine chloride Ray, Avik; Bhattacharya, Sabita An efficient method of micropropagation for Eclipta alba from young nodal axils of shoot tip explants has been developed by giving special attention to 'priming' in vitro plantlets in view of increasing their hardening ability after ransplantation ex vitro. Among 3 cytokinins-BAP, kinetin and TDZ, BAP was found most effective in inducing and proliferating adventitious shoots. The highest frequency of responding explants (100%) and maximum number of shoots (23.0) per explant were obtained after 60 days culture on MS medium containing 8.8 mu M BAP. Cent percent shoots developed roots directly from shoot base when transferred to growth regulator-free MS medium. For priming E. alba microshoots, 6.3 mu M of chlorocholine chloride (CCC) was found most effective. The major changes observed in 30 days old treated shoots were, production of increased number of root, elevation of chlorophyll level in leaves and increase in plant biomass. Furthermore, arrested undesirable shoot elongation made the plants sturdier and more suitable for acclimatization. The primed micropropagated E. alba plants were healthy and survived by higher frequency (100%) in soil in comparison to the non-treated plants (84% survival). DOI: 10.1007/s11240-007-9328-y Sat, 01 Mar 2008 00:00:00 GMT http://192.168.1.40:8080/xmlui/handle/123456789/1983 2008-03-01T00:00:00Z