http://192.168.1.40:8080/xmlui/handle/123456789/1142 Sun, 31 May 2026 14:24:28 GMT 2026-05-31T14:24:28Z http://192.168.1.40:80/xmlui/bitstream/id/6aba98fd-74fe-48db-b83f-beeac6e25e7e/DB1.jpg http://192.168.1.40:8080/xmlui/handle/123456789/1142 http://192.168.1.40:8080/xmlui/handle/123456789/2418 Targeted activation tagging of the Arabidopsis NBS-LRR gene, ADR1, conveys resistance to virulent pathogens Grant, J.J.; Chini, A.; Basu, Debabrata; Loake, G.J. A transgenic Arabidopsis line containing a chimeric PR-1::luciferase (LUC) reporter gene was subjected to mutagenesis with activation tags. Screening of lines via high-throughput LUC imaging identified a number of dominant Arabidopsis mutants that exhibited enhanced PR-1 gene expression. Here, we report the characterization of one of these mutants, designated activated disease resistance (adr) 1. This line showed constitutive expression of a number of key defense marker genes and accumulated salicylic acid but not ethylene or jasmonic acid. Furthermore, adr1 plants exhibited resistance against the biotrophic pathogens Peronospora parasitica and Erysiphe cichoracearum but not the necrotrophic fungus Botrytis cinerea. Analysis of a series of adr1 double mutants suggested that adr1-mediated resistance against P parasitica was salicylic acid (SA)-dependent, while resistance against E. cichoracearum was both SA-dependent and partially NPR1-dependent. The ADR1 gene encoded a protein possessing a number of key features, including homology to subdomains of protein kinases, a nucleotide binding domain, and leucine-rich repeats. The controlled, transient expression of ADR1 conveyed striking disease resistance in the absence of yield penalty, highlighting the potential utility of this gene in crop protection. DOI: 10.1094/MPMI.2003.16.8.669 Fri, 01 Aug 2003 00:00:00 GMT http://192.168.1.40:8080/xmlui/handle/123456789/2418 2003-08-01T00:00:00Z http://192.168.1.40:8080/xmlui/handle/123456789/2353 The mannitol cycle in Pleurotus ostreatus (Florida) Chakraborty, T. K.; Basu, Debabrata; Das, N.; Sengupta, S.; Mukherjee, M. In mushroom, presence of the mannitol cycle has not been reported so far although the polyol is supposed to be generated by the reduction of fructose by mannitol dehydrogenase. This study submits evidence for the presence of the mannitol cycle in Pleurotus ostreatus. The key enzyme of the cycle, mannitol-1-phosphate dehydrogenase (M1PDH), was present appreciably in all the developmental stages of the mushroom. However, the enzyme level dropped significantly at the onset of sporulation. The presence of M1DPH was confirmed by isozyme analysis and RT-PCR mediated amplification of a similar to400 bp DNA fragment. (C) 2004 Federation of European Microbiological Societies. DOI: 10.1016/j.femsle.2004.06.002 Thu, 15 Jul 2004 00:00:00 GMT http://192.168.1.40:8080/xmlui/handle/123456789/2353 2004-07-15T00:00:00Z http://192.168.1.40:8080/xmlui/handle/123456789/2089 PTC-1: A homologue of TFL1/CEN involved in the control of shoot architecture in Beta palonga Datta, Siraj; Basu, Debabrata; Mukherjee, Kalyan Kumar A terminal flower mutant phenotype of Beta palonga, a leafy vegetable, isolated earlier has been characterized. Micromorphological and histological study of the normal and mutant phenotype reveals physiognomic similarity of the mutant phenotype with terminal flower mutant of Arabidopsis. To investigate the regulation of meristem identity and control of flower development of the mutant phenotype, degenerated primers were designed from the highly conserved region of floral identity gene TFL1/CEN. This resulted in a 238 base pair-specific amplified cDNA product by RT-PCR, named as PTC-1. Sequence analysis followed by BLAST showed high homology of the PTC with TFL1/CEN-like gene, indicating the presence of TFL1/CEN homologue in B. palonga. Southern analysis indicates alteration of the genomic sequence of the mutant of B. palonga. The significance of the terminal flower is discussed. Thu, 10 Jan 2008 00:00:00 GMT http://192.168.1.40:8080/xmlui/handle/123456789/2089 2008-01-10T00:00:00Z http://192.168.1.40:8080/xmlui/handle/123456789/1968 Differential profiling of selected defence-related genes induced on challenge with Alternaria brassicicola in resistant white mustard and their comparative expression pattern in susceptible India mustard Ghose, Kaushik; Dey, Sanjukta; Barton, Hannah; Loake, Gary J; Basu, Debabrata The lack of availability of sources of resistance against Alternaria brassicicola within the family Brassicaceae has made oilseed mustard plants a target for one of the most damaging and widespread fungal diseases, Alternaria black spot. Of the other non-host-resistant/tolerant plants, Sinapis alba, white mustard, is considered to be the most important apart from Arabidopsis. To understand the defence response of S. alba upon incompatible interaction with this pathogen, a functional genomic approach using cDNA amplified fragment length polymorphism was performed. The highly reproducible bands, found to be either more amplified or uniquely present in infected S. alba plants compared with non-infected plants, were further subjected to comparative reverse Northern analysis in the incompatible white mustard (S. alba) and compatible India mustard (Brassica juncea L.) plants. The suppression of 46% of the genes in the compatible background indicates the possibility of effective and specific recognition of Alternaria in S. alba. Analysis of the 118 genes up-regulated specifically in infected S. alba compared with B. juncea showed that 98 genes have similarity to proteins such as receptor-like protein kinase genes, genes involved with calcium-mediated signalling and salicylic acid-dependent genes as well as other genes of known function in Arabidopsis. The apparent expression profile data were further confirmed for selected genes by quantitative real-time polymerase chain reaction analysis. Classification of these genes on the basis of their induction pattern in Arabidopsis indicates that the expression profile of several of these genes was distinct in S. alba compared with B. juncea. DOI: 10.1111/J.1364-3703.2008.00497.X Sat, 01 Nov 2008 00:00:00 GMT http://192.168.1.40:8080/xmlui/handle/123456789/1968 2008-11-01T00:00:00Z