http://192.168.1.40:8080/xmlui/handle/123456789/1143 Sun, 31 May 2026 14:24:32 GMT 2026-05-31T14:24:32Z http://192.168.1.40:80/xmlui/bitstream/id/b33fadeb-a90e-497b-afed-0229673246e9/ggn.jpg http://192.168.1.40:8080/xmlui/handle/123456789/1143 http://192.168.1.40:8080/xmlui/handle/123456789/2435 Luffa sponge - a unique matrix for tissue culture of Philodendron Gangopadhyay, Gaurab; Bandyopadhyay, T.; Gangopadhyay, S. B.; Mukherjee, K. K. An efficient and simple micropropagation protocol of Philodendron 'Xanadu', highly esteemed for its beautiful foliage, has been established. Rapid multiplication was attained by high concentration of N-6-benzyladenine, but a sequential lowering of the same was required for obtaining healthy plantlets. A study on in vitro root development in conventional agar-gelled media in comparison to liquid media with two alternative matrices like Luffa sponge and coir indicated the suitability of Luffa sponge over the others. The characteristic pattern of leaf morphology though was absent in the micropropagated plants initially, but the notches and crevices of the lamina appeared gradually with age after transplantation to soil. RAPD profiles of the randomly selected clones of different age in comparison to the mother plant were identical, thus assuring a totally genetic fidelity-maintained protocol for this commercially important plant. Sun, 25 Jan 2004 00:00:00 GMT http://192.168.1.40:8080/xmlui/handle/123456789/2435 2004-01-25T00:00:00Z http://192.168.1.40:8080/xmlui/handle/123456789/2432 Micropropagation of Tectona grandis: assessment of genetic fidelity Gangopadhyay, Gaurab; Basu Gangopadhyay, S.; Poddar, R.; Gupta, S.; Mukherjee, K. K. Random amplified polymorphic DNA (RAPD) markers were used to analyze genetic fidelity of micropropagated teak (Tectona grandis L.) clones with respect to subcultural passage. Of the twenty primers screened, no variation in RAPD profiles was noticed in the in vitro clones of fifth, tenth, fifteenth and twentieth passage in comparison to the in vivo mother plants. Only one micropropagated plant of twenty-fifth subcultural passage, however, differed from the in vivo ones. It revealed the appearance of a new polymorphic DNA fragment (molecular mass 379 kb) in case of primer OPB-08. This primer, manifesting detectable variation, may be utilized as a diagnostic marker for assessing genetic fidelity of micropropagted teak plants. DOI: 10.1023/A:1024359126802 Wed, 01 Jan 2003 00:00:00 GMT http://192.168.1.40:8080/xmlui/handle/123456789/2432 2003-01-01T00:00:00Z http://192.168.1.40:8080/xmlui/handle/123456789/2422 Agrobacterium-mediated genetic transformation in Indian spinach (Beta palonga) Gangopadhyay, Gaurab; Bandyopadhyay, T.; Datta, S.; Basu, D.; Mukherjee, K.K. An efficient and reproducible protocol for Agrobacterium-mediated plant transformation has been standardized in Indian spinach (Beta palonga) with a standard vector (pCambia 1304). Passage wise subculture in medium containing hygromycin, the selection agent, eventually resulted in survival of two percent (eleven in number) of originally infected explants showing continuous organogenesis. Of those eleven lines only three showed GUS amplification after PCR analysis followed by GUS positive histochemical assay. Those three confirmed transgenics were multiplied followed by successful rooting in liquid medium with coir as alternative matrix. Mon, 01 Sep 2003 00:00:00 GMT http://192.168.1.40:8080/xmlui/handle/123456789/2422 2003-09-01T00:00:00Z http://192.168.1.40:8080/xmlui/handle/123456789/2344 Trichomes: their diversity, distribution and density in acclimatization of teak (Tectona grandis L.) plants grown in vitro Bandyopadhyay, T.; Gangopadbyay, Gaurab; Poddar, Ramit.; Mukherjee, K. K. Diversity, distribution and density of trichomes were studied in relation to other morphological and physiological parameters of in vivo and in vitro teak (Tectona grandis L.) plants with the objective to understand their role in hardening and acclimatization of micropropagated plants. Less diversity and scanty distribution of trichomes of in vitro leaves in comparison to the in vivo ones were correlated with water loss of the former plants leading to low rate of survival after transplantation. The probable cause for absence of certain types of trichome in in vitro plants was also discussed. DOI: 10.1023/B:TICU.0000022534.03276.c5 Sun, 01 Aug 2004 00:00:00 GMT http://192.168.1.40:8080/xmlui/handle/123456789/2344 2004-08-01T00:00:00Z