Prof. K .P. Das
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Recent Submissions
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Purification and characterization of α-amylase from Bacillus amyloliquefaciens NCIM 2829
(NATL INST SCIENCE COMMUNICATION, 2005-10-01)α-Amylase (EC 3.2.1.1) was purified to homogeneity (specific activity 58,000 μmole min -1 mg protein -1) from the culture filtrate of Bacillus amyloliquefaciens NCIM 2829. Its molecular mass was found to be 67.5 kDa. The ... -
Accessibility of the various regions of β-lactoglobulin during thermal unfolding and refolding
(Indian Society for Surface Science and Technology, 2004)Structure of the refolded β-lactoglobulin has been compared with that of native β-lactoglobulin by measuring the accessibility of the various regions of the protein molecule. Site selective fluorescence labelling was ... -
An insight into the molecular basis of salt tolerance of L-myo-inositol 1-P synthase (PcINO1) from Porteresia coarctata (Roxb.) Tateoka, a halophytic wild rice
(AMER SOC PLANT BIOLOGISTS, 2006-04-01)The molecular basis of salt tolerance of L myo-inositol 1-P synthase (MIPS; EC 5.5.1.4) from Porteresia coarctata (Roxb.) Tateoka (PcINO1, AF412340) earlier reported from this laboratory, has been analyzed by in vitro ... -
Surface activity coefficients of spread monolayers of behenic acid salts at air-water interface
(ELSEVIER SCIENCE BV, 2006-11-16)The pressure-area isotherms of ionized monolayers of behenic acid at air-water interface at pH 12.0 have been obtained from the Langmuir film balance experiments under various physico-chemical conditions. The value of the ... -
Excess adsorption on hydrophobic and hydrophilic solid-liquid interfaces: Negative adsorption of electrolytes and urea. Part 2
(NATL INST SCIENCE COMMUNICATION, 2006-12-01)The extent of hydration of charcoal, cationic and anionic resin powders in the presence and absence of some inorganic salts and urea has been studied using isopiestic vapour pressure technique. The shapes of water vapour ... -
Excess adsorption on hydrophobic and hydrophilic solid-liquid interfaces. Positive excess adsorption of non-ionic surfactant. Part 1
(NATL INST SCIENCE COMMUNICATION, 2006-12-01)The extent of adsorption (Gamma(1)(2)) of non-ionic surfactant Tween-20 onto hydrophilic alumina and silica and hydrophobic charcoal surfaces has been measured as a function of surfactant concentration (X-2) in mole fraction ... -
Preparation of dibasic acid-containing soy phospholipids by chemical and enzyme-catalyzed transesterification and evaluation of their surface-active properties
(AMER OIL CHEMISTS SOC A O C S PRESS, 2006-12-01)The preparation of dibasic acid-containing soy phospholipid was made by transesterification reaction with alkyl ester of dibasic acid with both lipase and alkoxide as a catalyst. The extent of incorporation of a dibasic ... -
Differential recognition of natural and nonnatural substrate by molecular chaperone α-crystallin - A subunit exchange study
(JOHN WILEY & SONS INC, 2007-02-05)alpha-Crystallin is a molecular chaperone that recognizes proteins substrates in stress. It binds to the unstable conformer of a large variety of related or unrelated substrates and thus prevents them aggregating and holds ... -
Chaperone-mediated inhibition of tubulin self-assembly
(Elsevier B.V., 2007-04-01)Molecular chaperones are known to play an important role in facilitating the proper folding of many newly synthesized proteins. Here, we have shown that chaperone proteins exhibit another unique property to inhibit tubulin ... -
Methyl glyoxal elevation is associted with oxidative stress in rheumatoid arthritis
(TAYLOR & FRANCIS LTD, 2007)Methyl glyoxal (MG), a metabolic hazard plays a role in pathogenesis of different diseases. We studied the role of MG in cellular oxidative and carbonyl stress in rheumatoid arthritis (RA). 148 RA patients were divided ... -
Zn2+ enhances the molecular chaperone function and stability of α-crystallin
(ELSEVIER, 2008-01-15)α-Crystallin, the major eye lens protein, is a molecular chaperone that plays a crucial role in the suppression of protein aggregation and thus in the long-term maintenance of lens transparency. Zinc is a micronutrient of ... -
α-Crystallin assisted refolding of enzyme substrates: Optimization of external parameters
(SPRINGER, 2007-06-01)α-Crystallin is known to act as a molecular chaperone by preventing the aggregation of partially unfolded substrate proteins. It is also known to assist the refolding of a number of denatured enzymes, but the activity yield ... -
Unfolding and refolding of bovine α-crystallin in urea and its chaperone activity
(SPRINGER, 2007-08-01)We undertook an unfolding and refolding study of alpha(L)-crystallin in presence of urea to explore the breakdown and formation of various levels of structure and to find out whether the breakdown of various levels of ... -
Loss of structural integrity and hydrophobic ligand binding capacity of acetylated and succinylated bovine beta-lactoglobulin
(ELSEVIER, 2009-01)The lysine residues of bovine beta-lactoglobulin (beta-lg) were acetylated and succinylated to investigate the effect of chemical modification on tertiary and secondary structures. Both derivatives showed higher electrophoretic ... -
Water vapour adsorption by L-amino acids at different water activities
(SCIENTIFIC PUBL-INDIA, 2008-12)Experimental studies on the hydration of all 20 L-amino acids by isopiestic vapour pressure method have been carried out in detail. The water vapour adsorption curves of these amino acids resemble type It or type III BET ... -
Unfolding Studies of Escherichia coli Maltodextrin Glucosidase Monitored by Fluorescence Spectroscopy
(SPRINGER, 2008-12)Equilibrium unfolding of a 69-kDa monomeric Escherichia coli maltodextrin, glucosidase (MalZ) was studied using intrinsic and extrinsic fluorescence spectroscopy. The unfolding transition of MaIZ followed a three-state ... -
Excess adsorption of beta-lactoglobulin on the soft surface of casein powder
(SCIENTIFIC PUBL-INDIA, 2010-02)Extents (Gamma(1)(2)) of adsorption or globular milk protein beta-lactoglobulin (beta lg) from salt solution onto the soft surface of casein powder have been measured as functions or beta lg concentration at pH 6.5 keeping ... -
Effect of Methylglyoxal Modification of Human alpha-Crystallin on the Structure, Stability and Chaperone Function
(SPRINGER, 2010-11)alpha-Crystallin functions as a molecular chaperone and maintains transparency of eye lens by protecting other lens-proteins. Non-enzymatic glycation of alpha-crystallin by methylglyoxal, plays a crucial role on its chaperone ... -
AtPoiA, a homogue of mammalian DNA polymerase in Arabidopsis thaliana, is involved in the repair of UV-B induced DNA damage through the dark repair pathway
(OXFORD UNIV PRESS, 2011-02)Plants are constantly exposed to a wide range of environmental genotoxic stress factors including obligatory exposure to UV radiation in sunlight. Here, we report the functional characterization of a DNA repair protein, ... -
Stabilization of oligomeric structure of α-crystallin by Zn+2 through intersubunit bridging
(WILEY ONLINE LIBRARY, 2010-09-20)α-Crystallin, the major protein of mammalian eye lens, is a member of the small heat shock protein family and is a molecular chaperone. We previously reported that its molecular chaperone function as well as stability ...
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