Prof. Anuradha Lohia

The genome of the protist parasite Entamoeba histolytica

Loftus, B.; Lohia, Anuradha (NATURE PUBLISHING GROUP, 2005-02-24)

Entamoeba histolytica is an intestinal parasite and the causative agent of amoebiasis, which is a significant source of morbidity and mortality in developing countries(1). Here we present the genome of E. histolytica, which ...

Entamoeba histolytica DNA methyltransferase (Ehmeth) is a nuclear matrix protein that binds EhMRS2, a DNA that includes a scaffold/matrix attachment region (S/MAR)

Banerjee, S.; Fisher, O.; Lohia, Anuradha; Ankri, S. (ELSEVIER SCIENCE BV, 2005-01)

The protozoan parasite Entamoeba histolytica express a cytosine-5 DNA methyltransferase (Ehmeth) that belongs to the DNMT2 protein family. The biological function of members of this DNMT2 family is unknown. In the present ...

Inhibition of gene expression with double strand RNA interference in Entamoeba histolytica

Kaur, G.; Lohia, Anuradha (ACADEMIC PRESS INC ELSEVIER SCIENCE, 2004-08-06)

In order to inhibit gene expression in Entamoeba histolytica, we have developed a method based on expressing double strand RNA interference constructs in stable transformants. The 5' end of Eh Dia was cloned head to head ...

The Iml3 protein of the budding yeast is required for the prevention of precocious sister chromatid separation in meiosis I and for sister chromatid disjunction in meiosis II

Ghosh, S. K.; Sau, S.; Lahiri, S.; Lohia, Anuradha; Sinha, Pratima (SPRINGER, 2004-08)

The mitotic kinetochore of the budding yeast contains a number of proteins which are required for chromosome transmission but are non-essential for vegetative growth. We show that one such protein, Iml3, is essential for ...

Sequence divergence of Entamoeba histolytica tubulin is responsible for its altered tertiary structure

Roy, D.; Lohia, Anuradha (ACADEMIC PRESS INC ELSEVIER SCIENCE, 2004-07-02)

Atypical microtubular structures of the protozoan parasite Entamoeba histolytica (Eh) have been attributed to amino acid sequence divergence of Eh tubulin. To investigate if this sequence divergence leads to significant ...

Leishmanial proteins can correct human mitochondrial mutations

Lohia, Anuradha (INDIAN ACADEMY SCIENCES, 2006-12)

Eh Klp5 is a divergent member of the kinesin 5 family that regulates genome content and microtubular assembly in Entamoeba histolytica

Dastidar, Promita Ghosh; Majumder, Shubhra; Lohia, Anuradha (WILEY-BLACKWELL, 2007-02)

Earlier studies have established two unusual features in the cell division cycle of Entamoeba histolytica. First, microtubules form a radial assembly instead of a bipolar mitotic spindle, and second, the genome content of ...

EhRho1, a RhoA-like GTPase of Entamoeba histolytica, is modified by clostridial glucosylating cytotoxins

Majumder, Shubhra; Schmidt, Gudula; Lohia, Anuradha; Aktories, Klaus (AMER SOC MICROBIOLOGY, 2006-12)

Clostridial glucosylating cytotoxins inactivate mammalian Rho GTPases by mono-O glucosylation of a conserved threonine residue located in the switch 1 region of the target protein. Here we report that EhRho1, a RhoA-like ...

Bipolar spindle frequency and genome content are inversely regulated by the activity of two N-type kinesins in Entamoeba histolytica

Dastidar, Promita Ghosh; Lohia, Anuradha (WILEY-BLACKWELL, 2008-06)

Bipolar microtubular spindles are seen infrequently in Entamoeba histolytica trophozoites while monopolar or radial microtubular assemblies are common. Additionally, heterogeneity in nuclear DNA content and multi-nucleation ...

Entamoeba histolytica encodes unique formins, a subset of which regulates DNA content and cell division

Majumder, Shubhra; Lohia, Anuradha (AMER SOC MICROBIOLOGY, 2008-06)

The formin family of proteins mediates dynamic changes in actin assembly in eukaryotes, and therefore it is important to understand the function of these proteins in Entamoeba histolytica, where actin forms the major ...

Genome re-duplication and irregular segregation occur during the cell cycle of Entamoeba histolytica

Lohia, Anuradha; Mukherjee, Chandrama; Majumder, Shubhra; Dastidar, Promita Ghosh (SPRINGER/PLENUM PUBLISHERS, 2007-12)

Heterogeneity of genome content is commonly observed in axenic cultures of Entamoeba histolytica. Cells with multiple nuclei and nuclei with heterogenous genome contents suggest that regulatory mechanisms that ensure ...

Inter-Cellular Variation in DNA Content of Entamoeba histolytica Originates from Temporal and Spatial Uncoupling of Cytokinesis from the Nuclear Cycle

Mukherjee, Chandrama; Majumder, Shubhra; Lohia, Anuradha (PUBLIC LIBRARY SCIENCE, 2009-04)

Accumulation of multiple copies of the genome in a single nucleus and several nuclei in a single cell has previously been noted in Entamoeba histolytica, contributing to the genetic heterogeneity of this unicellular ...