Metabolism of hexane by Rhizobium sp. (Cicer arietinum) BICC620

Abstract

Activities of key enzymes of the Embden Meyerhof Parnas-, EntnerDoudoroff-, and pentose phosphate-pathways as well as key enzymes for gluconeogenesis and tri carboxylic acid cycle were assayed from cell free extracts (CFE) of hexane grown culture of Rhizobium sp. (Cicer arietinum) BICC 620. Level of activities of the enzymes assayed indicate either a very poor or no operation of glycolytic pathways from the CFE, whereas, operation of TCA cycle was moderate as significant level of activities of key enzymes of the pathway is detected from hexane-grown cells of the strain. Cells also operate gluconeogenesis comparatively at a higher rate as indicated by the substantial specific activity fructose-1,6-bisphosphatase and malic enzyme from the CFE.