| dc.contributor.author | Gangopadhyay, Gaurab | |
| dc.contributor.author | Roy, Subhash Kanti | |
| dc.contributor.author | Gangopadhyay, Sangita Basu | |
| dc.contributor.author | Mukherjee, Kalyan Kumar | |
| dc.date.accessioned | 2012-11-27T09:26:45Z | |
| dc.date.available | 2012-11-27T09:26:45Z | |
| dc.date.issued | 2009-06 | |
| dc.identifier | FOR ACCESS / DOWNLOAD PROBLEM -- PLEASE CONTACT LIBRARIAN, BOSE INSTITUTE, akc@bic.boseinst.ernet.in | en_US |
| dc.identifier.citation | Gangopadhyay G, Roy S K, Basu .Gangopadhyay Sand Mukherjee K K (2009) Agrobacterium-mediated genetic transformation of pineapple var. Queen using a novel encapsulation-based antibiotic selection technique, Plant Cell Tissue Organ Culture, 97, 295-302. | en_US |
| dc.identifier.issn | 0167-6857 | |
| dc.identifier.uri | 1. Full Text Link -> | en_US |
| dc.identifier.uri | http://link.springer.com/article/10.1007%2Fs11240-009-9528-8?LI=true#page-1 | en_US |
| dc.identifier.uri | ================================================= | en_US |
| dc.identifier.uri | 2. Scopus : Citation Link -> | en_US |
| dc.identifier.uri | http://www.scopus.com/record/display.url?eid=2-s2.0-67349238600&origin=resultslist&sort=plf-f&src=s&st1=Agrobacterium-mediated+genetic+transformation+of+pineapple&sid=1QDLWuFfqnBXvGpi0zd8Ls9%3a980&sot=b&sdt=b&sl=73&s=TITLE-ABS-KEY%28Agrobacterium-mediated+genetic+transformation+of+pineapple%29&relpos=1&relpos=1&searchTerm=TITLE-ABS-KEY%28Agrobacterium-mediated%20genetic%20transformation%20of%20pineapple%29 | en_US |
| dc.description | DOI: 10.1007/s11240-009-9528-8 | en_US |
| dc.description.abstract | A protocol for Agrobacterium-mediated transformation of a local 'elite' Indian variety (Queen) of pineapple [Ananus comosus (L.) Merr, family Bromeliaceae] has been established using a standard transformation vector (pCAMBIA 1304). High transformation efficiency, expressed as the mean percentage of transgenic micro-shoots regenerated from initial callus explants (20.6%) was achieved using a novel encapsulation-based, antibiotic selection procedure. The Agrobacterium-infected micro-shoots derived from callus explants survived selection in high concentration of hygromycin (60 mg l(-1) and beyond) in encapsulated alginate beads. The integration of transgene in hygromycin-resistant shoots and plants was confirmed by histochemical GUS assay, PCR amplification and Southern hybridization. It is possible to eliminate false antibiotic positives in pineapple transformation program to a large extent following this procedure. | en_US |
| dc.language.iso | en | en_US |
| dc.publisher | SPRINGER | en_US |
| dc.subject | Encapsulation-based selection | en_US |
| dc.subject | Pineapple var. Queen | en_US |
| dc.subject | Transformation | en_US |
| dc.subject | WOS:000265942400008 | en_US |
| dc.title | Agrobacterium-mediated genetic transformation of pineapple var. Queen using a novel encapsulation-based antibiotic selection technique | en_US |
| dc.title.alternative | Plant Cell, Tissue and Organ Culture | en_US |
| dc.type | Article | en_US |
