In vitro plant regeneration from leaf sheath cultures of sugarcane via organogenesis

Abstract

vitro morphogenesis was obtained from leaf sheath derived calli of three local varieties (lsd - 16, lsd -28 and Amrita) of sugarcane Saccharum officinarum L). The calli induced in 2,4 - D (0.5mg/l) containing MS medium showed better regenerating capacity than NAA containing one. Genotype specific requirement of auxin for regeneration of shoots was observed in the three varietes of sugarcane. Shoot differentiation started from the first passage of subculture in regeneration medium, reached its peak at third passage but declined beyond fifth passage of subculture. Rooting was induced in MS medium supplemented with high concentration of auxin (5.0 mg/I) and sucrose (5%). Mass propagation of plantlets was attained through in vitro tillering in half strength of liquid MS medium.