Browsing Prof. Gautam Basu by Issue Date
Now showing items 1-11 of 11
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Metal binding to cowpea chlorotic mottle virus using terbium(III) fluorescence (M-T-2003-04)
(SPRINGER-VERLAG, 2003-09)Metals are thought to play a role in the structure of many viruses. The crystal structure of the T=3 icosahedral cowpea chlorotic mottle virus (CCMV) suggests the presence of 180 unique metal-binding sites in the assembled ... -
Expanded turn conformations: Characterization and sequence-structure correspondence in alpha-turns with implications in helix folding
(WILEY-BLACKWELL,, 2004-05-01)Like the beta-turns, which are characterized by a limiting distance between residues two positions apart (i, i+3), a distance criterion (involving residues at positions i and i+4) is used here to identify a-turns from a ... -
Electrostatic potential of nucleotide-free protein is sufficient for discrimination between adenine and guanine-specific binding sites
(ACADEMIC PRESS, 2004-09-17)Despite sharing many common features, adenine-binding and guanine-binding sites in proteins often show a clear preference for the cognate over the non-cognate ligand. We have analyzed electrostatic potential (ESP) patterns ... -
Biphasic kinetics of the colchicine-tubulin interaction: Role of amino acids surrounding the a ring of bound colchicine molecule
(AMER CHEMICAL SOC, 2005-08-02)Isotypes of vertebrate tubulin have variable amino acid sequences, which are clustered at their C-terminal ends. Isotypes bind colchicine at different on-rates and affinity constants. The kinetics of colchicine binding to ... -
Paramagnetic viral nanoparticles as potential high-relaxivity magnetic resonance contrast agents
(JOHN WILEY, 2005-10)In order to compensate for the inherent high threshold of detectability of MR contrast agents, there has been an active interest in the development of paramagnetic nanoparticles incorporating high payloads of Gd3+ with ... -
Cloning, expression, purification, and characterization of Vibrio cholerae transcriptional activator, HlyU
(ACADEMIC PRESS INC ELSEVIER SCIENCE, 2006-07)The HlyU from Vibrio cholerae, involved in the transcriptional regulation of haemolysin genes, plays an important role in the regulation of virulence gene expression. We have cloned, over-expressed and purified HlyU from ... -
A chimaeric glutamyl:glutaminyl-tRNA synthetase: implications for evolution
(PORTLAND, 2009-01-15)aaRSs (aminoacyl-tRNA synthetases) are multi-domain proteins that have evolved by domain acquisition. The anti-codon binding domain was added to the more ancient catalytic domain during aaRS evolution. Unlike in eukaryotes, ... -
Coulomb energies of protein-protein complexes with monopole-free charge distributions
(ELSEVIER SCIENCE, 2009-04)From an analysis of Coulomb energy distributions of a large set of protein-protein complexes we show that the positive tail in the energy distribution disappears when the monopole-monopole term, the only energy term ... -
The role of the catalytic domain of E. coli GluRS in tRNA(Gln) discrimination
(ELSEVIER SCIENCE, 2009-06-18)Discrimination of tRNAGln is an integral function of several bacterial glutamyl-tRNA synthetases (GluRS). The origin of the discrimination is thought to arise from unfavorable interactions between tRNAGln and the ... -
Conformational preferences of a short Aib/Ala-based water-soluble peptide as a function of temperature
(WILEY-BLACKWELL, 2009-07)The amino acid Aib predisposes a peptide to be helical with context-dependent preference for either 3(10)- or alpha- or a mixed helical conformation. Short peptides also show an inherent tendency to be unfolded. To ... -
Human telomere d[(TTAGGG)(4)] undergoes a conformational transition to the Na+-form upon binding with sanguinarine in presence of K+
(ACADEMIC PRESS INC ELSEVIER SCIENCE, 2011-01-07)Guanine-rich telomeric sequences fold into G-quadruplex conformation and are known to bind a variety of ligands including potential drug candidates. By means of CD spectroscopy and fluorescence lifetime measurements we ...