Dr. Subrata Sau
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The bacteriophage lambda DNA replication protein P inhibits the oriC DNA- and ATP-binding functions of the DNA replication initiator protein DnaA of Escherichia coli
(SPRINGER SINGAPORE PTE LTD, 2005-01-31)Under the condition of expression of ?, P protein at lethal level, the oriC DNA-binding activity is significantly affected in wild-type E. coli but not in the rpl mutant. In purified system, the 2, P protein inhibits the ... -
The mutation that makes Escherichia coli resistant to λ P gene-mediated host lethality is located within the DNA initiator gene dnaA of the bacterium
(SPRINGER SINGAPORE PTE LTD, 2005-01-31)Earlier, we reported that the bacteriophage lambda P gene product is lethal to Escherichia coli, and the E. coli rpl mutants are resistant to this lambda P gene-mediated lethality. In this paper, we show that under the ... -
A point mutation at the C-terminal half of the repressor of temperate mycobacteriophage L1 affects its binding to the operator DNA
(SPRINGER SINGAPORE PTE LTD, 2004-11-30)The wild-type repressor CI of temperate mycobacteriophage Ll and the temperature-sensitive (ts) repressor CIts391 of a mutant Ll phage, L1cIts391, have been separately overexpressed in E. coli. Both these repressors were ... -
Purification and characterization of a deoxyriboendonuclease from Mycobacterium smegmatis
(SPRINGER SINGAPORE PTE LTD, 2006-03-31)A deoxyriboendonuclease has been purified to near homogeneity from a fast growing mycobacterium species, M. smegmatis and characterized to some extent. The size of enzyme is about 43 kDa as determined by a denaturing gel ... -
Studies on codon usage in Thermoplasma acidophilum and its possible implications on the occurrences of lateral gene transfer
(WILEY-V C H VERLAG GMBH, 2005)Codon usage studies have been carried out on the coding sequences of Thermoplasma acidophilum, which is an archaeon and grows at very low pH and high temperature. Overall codon usage data analysis indicates that all the ... -
Synonymous codon usage bias in 16 Staphylococcus aureus phages: Implication in phage therapy
(ELSEVIER, 2005-11)To reveal the factors influencing architecture of protein-coding genes in staphylococcal phages, relative synonymous codon usage variation has been investigated in 920 protein-coding genes of 16 staphylococcal phages. As ... -
Comparative analysis of the base composition and codon usages in fourteen mycobacteriophage genomes
(ADENINE PRESS, 2005-08)To study the possible codon usage and base composition variation in the bacteriophages, fourteen mycobacteriophages were used as a model system here and both the parameters in all these phages and their plating bacteria, ... -
Factors influencing the synonymous codon and amino acid usage bias in AT-rich Pseudomonas aeruginosa phage PhiKZ
(BLACKWELL, 2005-09)To reveal how the AT-rich genome of bacteriophage PhiKZ has been shaped in order to carry out its growth in the GC-rich host Pseudomonas aeruginosa, synonymous codon and amino acid usage bias of PhiKZ was investigated and ... -
The G23 and G25 genes of temperate mycobacteriophage L1 are essential for the transcription of its late genes
(SPRINGER SINGAPORE PTE LTD, 2007-03-31)Two lysis-defective but DNA synthesis non-defective temperature-sensitive (is) mutants of mycobacteriophage L1, L1G23ts23 and L1G25ts889 were found to be defective also in phage-specific RNA synthesis in the late period ... -
Studies on synonymous codon and amino acid usage biases in the broad-host range bacteriophage KVP40(M-T-2007)
(MICROBIOLOGY SOC KOREA, 2007-02)In this study, the relative synonymous codon and amino acid usage biases of the broad-host range phage, KVP40, were investigated in an attempt to understand the structure and function of its proteins/proteincoding genes, ... -
Effects of physical, ionic, and structural factors on the binding of repressor of mycobacteriophage L1 to its cognate operator DNA
(BENTHAM SCIENCE PUBL LTD, 2006)To determine the factors influencing the binding of L1 repressor to its cognate operator DNA, several gel shift as well as bioinformatic analyses have been carried out. The data show that time, temperature, salt, and pH ... -
Factors influencing synonymous codon and amino acid usage biases in Mimivirus
(ELSEVIER SCI LTD, 2006-08)Synonymous codon and amino acid usage biases have been investigated in 903 Mimivirus protein-coding genes in order to understand the architecture and evolution of Mimivirus genome. As expected for an AT-rich genome, third ... -
Detection of antistaphylococcal and toxic compounds by biological assay systems developed with a reporter Staphylococcus aureus strain harboring a heat inducible promoter - lacZ transcriptional fusion
(SPRINGER SINGAPORE PTE LTD, 2007-11-30)Previously it was reported that promoter of groES-groEL operon of Staphylococcus aureus is induced by various cellwall active antibiotics. In order to exploit the above promoter for identifying novel antistaphylococcal ... -
Purification and characterization of repressor of temperate S-aureus phage phi 11(M-T-2007)
(SPRINGER SINGAPORE PTE LTD, 2007-09-30)To gain insight into the structure and function of repressor proteins of bacteriophages of gram-positive bacteria, repressor of temperate Staphylococcus aureus phage 11 was undertaken as a model system here and purified ... -
Repressor of temperate mycobacteriophage LI harbors a stable C-terminal domain and binds to different asymmetric operator DNAs with variable affinity (M-T-2007)
(BIOMED CENTRAL LTD, 2007-06-28)Background: Lysogenic mode of life cycle of a temperate bacteriophage is generally maintained by a protein called 'repressor'. Repressor proteins of temperate lambdoid phages bind to a few symmetric operator DNAs in order ... -
Overexpression of a delayed early gene hlg1 of temperate mycobacteriophage L1 is lethal to both M-smegmatis and E-coli
(KOREAN SOCIETY BIOCHEMISTRY & MOLECULAR BIOLOGY, 2008-05-31)Two genes of temperate mycobacteriophage L5, namely, gp63 and gp64, were hypothesized to be toxic to M. smegmatis. An identical L5 gp64 ortholog (designated hlg1) was cloned from homoimmune mycobacteriophage L1 and ... -
Inactivation of indispensable bacterial proteins by early proteins of bacteriophages: Implication in antibacterial drug discovery
(BENTHAM SCIENCE PUBL LTD, 2008-06)Bacteriophages utilize host bacterial cellular machineries for their own reproduction and completion of life cycles. The early proteins that phage synthesize immediately after the entry of their genomes into bacterial cells ... -
The delayed early gene G23 of temperate mycobacteriophage L1 regulates the expression of deoxyribonuclease, the product of another delayed early gene of the phage
(POLSKIE TOWARZYSTWO MIKROBIOLOGOW-POLISH SOCIETY OF MICROBIOLOGISTS, 2008)To get clues about the genes as well as the gene regulatory circuit controlling the lytic development of temperate mycobacteriophage L1, previously we screened several conditional lethal mutants of L1 and characterized ... -
Virulence plasmid of Aeromonas hydrophila induces macrophage apoptosis and helps in developing systemic infection in mice
(ACADEMIC PRESS, 2009-02)Pathogenic Aeromonas hydrophila Strain AO1 bears a 21 kb plasmid encoding several virulence determinants. Infection studies revealed that this isolate induced cytotoxicity in BALB/c mice splenic macrophages involving ... -
Moderately thermostable phage Phi 11 Cro repressor has novel DNA-binding capacity and physicochemical properties
(KOREAN SOCIETY BIOCHEMISTRY & MOLECULAR BIOLOGY, 2009-03-31)The temperate Staphylococcus aureus phage Phi 11 harbors cl and cro repressor genes similar to those of lambdoid phages. Using extremely pure Phi 11 Cro (the product of the Phi 11 cro gene) we demonstrated that this protein ...
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