Browsing Dr. Subrata Sau by Title
Now showing items 1-20 of 28
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Antagonistic effects Na＋ and Mg2＋ on the structure, function, and stability of mycobacteriophage L1 repressor
(KOREAN SOCIETY BIOCHEMISTRY & MOLECULAR BIOLOGY, 2009-05-31)Temperate mycobacteriophage L1 encodes an unusual repressor (CI) for regulating its lytic-lysogenic switching and, in contrast to the repressors of most temperate phages, it binds to multiple asymmetric operator DNAs. Here, ... -
Antibiotics, arsenate and H2O2 induce the promoter of Staphylococcus aureus cspC gene more strongly than cold
(WILEY-BLACKWELL, 2009-04)Proteins expressed by the bacterial cold shock genes are highly conserved at sequence level and perform various biological functions in both the cold-stressed and normal cells. To study the effects of various agents on the ... -
The bacteriophage lambda DNA replication protein P inhibits the oriC DNA- and ATP-binding functions of the DNA replication initiator protein DnaA of Escherichia coli
(SPRINGER SINGAPORE PTE LTD, 2005-01-31)Under the condition of expression of ?, P protein at lethal level, the oriC DNA-binding activity is significantly affected in wild-type E. coli but not in the rpl mutant. In purified system, the 2, P protein inhibits the ... -
Characterization of an unusual cold shock protein from Staphylococcus aureus
(WILEY-V C H VERLAG GMBH, 2010-12)Of the three cold shock proteins expressed by Staphylococcus aureus, CspC is induced poorly by cold but strongly by various antibiotics and toxic chemicals. Using a purified CspC, here we demonstrate that it exists as a ... -
Comparative analysis of the base composition and codon usages in fourteen mycobacteriophage genomes
(ADENINE PRESS, 2005-08)To study the possible codon usage and base composition variation in the bacteriophages, fourteen mycobacteriophages were used as a model system here and both the parameters in all these phages and their plating bacteria, ... -
The delayed early gene G23 of temperate mycobacteriophage L1 regulates the expression of deoxyribonuclease, the product of another delayed early gene of the phage
(POLSKIE TOWARZYSTWO MIKROBIOLOGOW-POLISH SOCIETY OF MICROBIOLOGISTS, 2008)To get clues about the genes as well as the gene regulatory circuit controlling the lytic development of temperate mycobacteriophage L1, previously we screened several conditional lethal mutants of L1 and characterized ... -
Detection of antistaphylococcal and toxic compounds by biological assay systems developed with a reporter Staphylococcus aureus strain harboring a heat inducible promoter - lacZ transcriptional fusion
(SPRINGER SINGAPORE PTE LTD, 2007-11-30)Previously it was reported that promoter of groES-groEL operon of Staphylococcus aureus is induced by various cellwall active antibiotics. In order to exploit the above promoter for identifying novel antistaphylococcal ... -
Detection of the cell wall-affecting antibiotics at sublethal concentrations using a reporter Staphylococcus aureus harboring drp35 promoter-lacZ transcriptional fusion
(KOREAN SOCIETY BIOCHEMISTRY & MOLECULAR BIOLOGY, 2010-07-31)Previously, various inhibitors of cell wall synthesis induced the drp35 gene of Staphylococcus aureus efficiently. To determine whether drp35 could be exploited in antistaphylococcal drug discovery, we cloned the promoter ... -
Effects of physical, ionic, and structural factors on the binding of repressor of mycobacteriophage L1 to its cognate operator DNA
(BENTHAM SCIENCE PUBL LTD, 2006)To determine the factors influencing the binding of L1 repressor to its cognate operator DNA, several gel shift as well as bioinformatic analyses have been carried out. The data show that time, temperature, salt, and pH ... -
Factors influencing synonymous codon and amino acid usage biases in Mimivirus
(ELSEVIER SCI LTD, 2006-08)Synonymous codon and amino acid usage biases have been investigated in 903 Mimivirus protein-coding genes in order to understand the architecture and evolution of Mimivirus genome. As expected for an AT-rich genome, third ... -
Factors influencing the synonymous codon and amino acid usage bias in AT-rich Pseudomonas aeruginosa phage PhiKZ
(BLACKWELL, 2005-09)To reveal how the AT-rich genome of bacteriophage PhiKZ has been shaped in order to carry out its growth in the GC-rich host Pseudomonas aeruginosa, synonymous codon and amino acid usage bias of PhiKZ was investigated and ... -
The G23 and G25 genes of temperate mycobacteriophage L1 are essential for the transcription of its late genes
(SPRINGER SINGAPORE PTE LTD, 2007-03-31)Two lysis-defective but DNA synthesis non-defective temperature-sensitive (is) mutants of mycobacteriophage L1, L1G23ts23 and L1G25ts889 were found to be defective also in phage-specific RNA synthesis in the late period ... -
Inactivation of indispensable bacterial proteins by early proteins of bacteriophages: Implication in antibacterial drug discovery
(BENTHAM SCIENCE PUBL LTD, 2008-06)Bacteriophages utilize host bacterial cellular machineries for their own reproduction and completion of life cycles. The early proteins that phage synthesize immediately after the entry of their genomes into bacterial cells ... -
Moderately thermostable phage Phi 11 Cro repressor has novel DNA-binding capacity and physicochemical properties
(KOREAN SOCIETY BIOCHEMISTRY & MOLECULAR BIOLOGY, 2009-03-31)The temperate Staphylococcus aureus phage Phi 11 harbors cl and cro repressor genes similar to those of lambdoid phages. Using extremely pure Phi 11 Cro (the product of the Phi 11 cro gene) we demonstrated that this protein ... -
The mutation that makes Escherichia coli resistant to λ P gene-mediated host lethality is located within the DNA initiator gene dnaA of the bacterium
(SPRINGER SINGAPORE PTE LTD, 2005-01-31)Earlier, we reported that the bacteriophage lambda P gene product is lethal to Escherichia coli, and the E. coli rpl mutants are resistant to this lambda P gene-mediated lethality. In this paper, we show that under the ... -
Overexpression of a delayed early gene hlg1 of temperate mycobacteriophage L1 is lethal to both M-smegmatis and E-coli
(KOREAN SOCIETY BIOCHEMISTRY & MOLECULAR BIOLOGY, 2008-05-31)Two genes of temperate mycobacteriophage L5, namely, gp63 and gp64, were hypothesized to be toxic to M. smegmatis. An identical L5 gp64 ortholog (designated hlg1) was cloned from homoimmune mycobacteriophage L1 and ... -
Physicochemical properties and distinct DNA binding capacity of the repressor of temperate Staphylococcus aureus phage phi 11
(WILEY-BLACKWELL PUB., 2009-04)The repressor protein and cognate operator DNA of any temperate Staphylococcus aureus phage have not been investigated in depth, despite having the potential to enrich the molecular biology of the staphylococcal system. ... -
A point mutation at the C-terminal half of the repressor of temperate mycobacteriophage L1 affects its binding to the operator DNA
(SPRINGER SINGAPORE PTE LTD, 2004-11-30)The wild-type repressor CI of temperate mycobacteriophage Ll and the temperature-sensitive (ts) repressor CIts391 of a mutant Ll phage, L1cIts391, have been separately overexpressed in E. coli. Both these repressors were ... -
Purification and characterization of a deoxyriboendonuclease from Mycobacterium smegmatis
(SPRINGER SINGAPORE PTE LTD, 2006-03-31)A deoxyriboendonuclease has been purified to near homogeneity from a fast growing mycobacterium species, M. smegmatis and characterized to some extent. The size of enzyme is about 43 kDa as determined by a denaturing gel ... -
Purification and characterization of repressor of temperate S-aureus phage phi 11(M-T-2007)
(SPRINGER SINGAPORE PTE LTD, 2007-09-30)To gain insight into the structure and function of repressor proteins of bacteriophages of gram-positive bacteria, repressor of temperate Staphylococcus aureus phage 11 was undertaken as a model system here and purified ...