Homodimeric Alkaline Phosphatase Located at Helicoverpa armigera Midgut, a Putative Receptor of Cry1Ac Contains alpha-GaINAc in Terminal Glycan Structure as Interactive Epitope
Abstract
Helicoverpa armigera causes massive yield loss of various crops globally. Bacillus thuringiensis coded Cry1Ac toxin is effective against the pest. Through the present investigation, an alkaline phosphatase type putative receptor of the toxin was identified in the target insect gut BBMV. Lectin-ligand immunoblot assay detected the presence of alpha-GaINAc residue at the nonreducing terminal of the glycan structure in the membrane bound HaALP protein which mediates the toxin-receptor interaction.
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- Prof. Sampa Das [6]