Dr. Subrata Sau: Recent submissions
Now showing items 21-28 of 28
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Antibiotics, arsenate and H2O2 induce the promoter of Staphylococcus aureus cspC gene more strongly than cold
(WILEY-BLACKWELL, 2009-04)Proteins expressed by the bacterial cold shock genes are highly conserved at sequence level and perform various biological functions in both the cold-stressed and normal cells. To study the effects of various agents on the ... -
Antagonistic effects Na＋ and Mg2＋ on the structure, function, and stability of mycobacteriophage L1 repressor
(KOREAN SOCIETY BIOCHEMISTRY & MOLECULAR BIOLOGY, 2009-05-31)Temperate mycobacteriophage L1 encodes an unusual repressor (CI) for regulating its lytic-lysogenic switching and, in contrast to the repressors of most temperate phages, it binds to multiple asymmetric operator DNAs. Here, ... -
Stabilization of the primary sigma factor of Staphylococcus aureus by core RNA polymerase
(KOREAN SOCIETY BIOCHEMISTRY & MOLECULAR BIOLOGY, 2010-03-31)The primary sigma factor (sigma(A)) of Staphylococcus aureus, a potential drug target, was little investigated at the structural level. Using an N-terminal histidine-tagged sigma(A) (His-sigma(A)), here we have demonstrated ... -
Physicochemical properties and distinct DNA binding capacity of the repressor of temperate Staphylococcus aureus phage phi 11
(WILEY-BLACKWELL PUB., 2009-04)The repressor protein and cognate operator DNA of any temperate Staphylococcus aureus phage have not been investigated in depth, despite having the potential to enrich the molecular biology of the staphylococcal system. ... -
Staphylococcus aureus ClpC Divergently Regulates Capsule via sae and codY in Strain Newman but Activates Capsule via codY in Strain UAMS-1 and in Strain Newman with Repaired saeS
(AMER SOC MICROBIOLOGY, 2011-02)ClpC is an ATPase chaperone found in most Gram-positive low-GC bacteria. It has been recently reported that ClpC affected virulence gene expression in Staphylococcus aureus. Here we report that ClpC regulates transcription ... -
Detection of the cell wall-affecting antibiotics at sublethal concentrations using a reporter Staphylococcus aureus harboring drp35 promoter-lacZ transcriptional fusion
(KOREAN SOCIETY BIOCHEMISTRY & MOLECULAR BIOLOGY, 2010-07-31)Previously, various inhibitors of cell wall synthesis induced the drp35 gene of Staphylococcus aureus efficiently. To determine whether drp35 could be exploited in antistaphylococcal drug discovery, we cloned the promoter ... -
Regions and Residues of an Asymmetric Operator DNA Interacting with the Monomeric Repressor of Temperate Mycobacteriophage L1
(AMER CHEMICAL SOC, 2010-05-18)Previously, the repressor protein of mycobacteriophage L1 bound to two operator DNAs with dissimilar affinity. Surprisingly, the putative operator consensus sequence, 5'GGTGGa/cTGTCAAG, lacks the dyad symmetry reported for ... -
Characterization of an unusual cold shock protein from Staphylococcus aureus
(WILEY-V C H VERLAG GMBH, 2010-12)Of the three cold shock proteins expressed by Staphylococcus aureus, CspC is induced poorly by cold but strongly by various antibiotics and toxic chemicals. Using a purified CspC, here we demonstrate that it exists as a ...