DSpace at Bose Institute: Recent submissions
Now showing items 1721-1740 of 2749
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Properties of HflX, an Enigmatic Protein from Escherichia coli
(AMER SOC MICROBIOLOGY, 2009-04-01)The Escherichia coli gene hflX was first identified as part of the hflA operon, mutations in which led to an increased frequency of lysogenization upon infection of the bacterium by the temperate coliphage lambda. Independent ... -
Entamoeba Shows Reversible Variation in Ploidy under Different Growth Conditions and between Life Cycle Phases
(WILEY-BLACKWELL, 2010-07)We have discovered four sirtuin genes in Entamoeba histolytica, two of which are similar to eukaryotic sirtuins and two to bacterial and archaeal sirtuins. The eukaryotic sirtuin homolog, EhSir2a showed NAD+ dependent ... -
Concise Synthesis of a Hexasaccharide Related to the Adhesin Receptor of Streptococcus oralis ATCC 55229
(TAYLOR & FRANCIS, 2009-09)A concise synthesis of a hexasaccharide related to the adhesin receptor of Streptococcus oralis ATCC 55229 (previously characterized as Streptococcus sanguis H1) has been achieved in excellent yield. A general glycosylation ... -
Stabilization of the primary sigma factor of Staphylococcus aureus by core RNA polymerase
(KOREAN SOCIETY BIOCHEMISTRY & MOLECULAR BIOLOGY, 2010-03-31)The primary sigma factor (sigma(A)) of Staphylococcus aureus, a potential drug target, was little investigated at the structural level. Using an N-terminal histidine-tagged sigma(A) (His-sigma(A)), here we have demonstrated ... -
CXC Chemokine-Mediated Protection against Visceral Leishmaniasis: Involvement of the Proinflammatory Response
(UNIV CHICAGO PRESS, 2009-10-15)Visceral leishmaniasis, caused by the protozoan parasite Leishmania donovani, is characterized by the loss of ability of the host to generate an effective immune response. In the present study, the comparative potential ... -
Physicochemical properties and distinct DNA binding capacity of the repressor of temperate Staphylococcus aureus phage phi 11
(WILEY-BLACKWELL PUB., 2009-04)The repressor protein and cognate operator DNA of any temperate Staphylococcus aureus phage have not been investigated in depth, despite having the potential to enrich the molecular biology of the staphylococcal system. ... -
Novel Role of Phosphorylation-Dependent Interaction between FtsZ and FipA in Mycobacterial Cell Division
(PUBLIC LIBRARY SCIENCE, 2010-01-06)The bacterial divisome is a multiprotein complex. Specific protein-protein interactions specify whether cell division occurs optimally, or whether division is arrested. Little is known about these protein-protein interactions ... -
Novel role of Wag31 in protection of mycobacteria under oxidative stress
(WILEY-BLACKWELL, 2009-07)P>Wag31 of Mycobacterium tuberculosis belongs to the DivIVA family of proteins known to regulate cell morphology in Gram-positive bacteria. Here we demonstrate an unrecognized, novel role of Wag31 in oxidatively stressed ... -
Cavities in protein–DNA and protein–RNA interfaces
(OXFORD UNIV PRESS, 2009-08)An analysis of cavities present in protein-DNA and protein-RNA complexes is presented. In terms of the number of cavities and their total volume, the interfaces formed in these complexes are akin to those in transient ... -
Accessibility and partner number of protein residues, their relationship and a webserver, ContPlot for their display
(BIOMED CENTRAL, 2009-04-08)Background: Depending on chemical features residues have preferred locations – interior or exterior – in protein structures, which also determine how many other residues are found around them. The close packing of residues ... -
Structural and unfolding features of HlyT, a tetrameric LysR type transcription regulator of Vibrio cholerae
(ELSEVIER SCIENCE BV, 2009-08)HlyT from Vibrio cholerae is a positive regulator of Na+/H+ antiporter, important for the survival of the organism in an aquatic environment and within the human host. Here we report cloning, over-expression and purification ... -
RseA, the SigE specific anti-sigma factor of Mycobacterium tuberculosis, is inactivated by phosphorylation-dependent ClpC1P2 proteolysis
(WILEY-BLACKWELL, 2010-02)Central to the response of Mycobacterium tuberculosis to environmental stress is the regulation of genes under the control of alternative sigma factors. Sigma E of M. tuberculosis plays an important role in the intracellular ... -
Multifocal signal modulation therapy of cancer: ancient weapon, modern targets
(SPRINGER, 2010-03)Although safe in most cases, ancient treatments are ignored because neither their active components nor their molecular targets are well defined. This is not the case, however, with curcumin, a yellow-pigment substance and ... -
Theaflavins retard human breast cancer cell migration by inhibiting NF-kappa B via p53-ROS cross-talk
(ELSEVIER SCIENCE, 2010-01-04)The present study demonstrates that theaflavins exploit p53 to impede metastasis in human breast cancer cells. Our data suggest that p53-dependent reactive oxygen species (ROS) induce p53-phosphorylation via p38MAPK in a ... -
Empirical estimation of the energetic contribution of individual interface residues in structures of protein-protein complexes
(SPRINGER, 2009-09)We report a simple algorithm to scan interfaces in protein-protein complexes for identifying binding 'hot spots'. The change in side-chain solvent accessible area (ΔASA) of interface residues has been related to change in ... -
Tumor-Shed PGE(2) Impairs IL2R gamma c-Signaling to Inhibit CD4(+) T Cell Survival: Regulation by Theaflavins
(PUBLIC LIBRARY SCIENCE, 2009-10-08)Background: Many tumors are associated with decreased cellular immunity and elevated levels of prostaglandin E2 (PGE2), a known inhibitor of CD4+ T cell activation and inducer of type-2 cytokine bias. However, the role of ... -
Structure and Activity of Lysozyme on Binding to ZnO Nanoparticles
(AMER CHEMICAL SOC, 2010-03-02)The interaction between ZnO nanoparticles (NPs) and lysozyme has been studied using calorimetric as well as spectrophotometric techniques, and interpreted in terms of the three-dimensional structure. The circular ... -
Finding the optimum activation energy in DNA breathing dynamics: a simulated annealing approach
(IOP PUBLISHING, 2009-08-21)We demonstrate how the stochastic global optimization scheme of simulated annealing can be used to evaluate optimum parameters in the problem of DNA breathing dynamics. The breathing dynamics is followed in accordance with ... -
Dissection, residue conservation, and structural classification of protein-DNA interfaces
(WILEY-LISS, 2009-02-15)The basic DNA-binding modules of 128 protein-DNA interfaces have been analyzed. Although these are less planar, like the protein-protein interfaces, the protein-DNA interfaces can also be dissected into core regions in ... -
A model for signal transduction during quorum sensing in Vibrio harveyi
(IOP PUBLISHING, 2009-12)We present a framework for analyzing luminescence regulation during quorum sensing in the bioluminescent bacterium Vibrio harveyi. Using a simplified model for signal transduction in the quorum sensing pathway, we identify ...