Department of Biochemistry: Recent submissions
Now showing items 61-80 of 237
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Purification and characterization of repressor of temperate S-aureus phage phi 11(M-T-2007)
(SPRINGER SINGAPORE PTE LTD, 2007-09-30)To gain insight into the structure and function of repressor proteins of bacteriophages of gram-positive bacteria, repressor of temperate Staphylococcus aureus phage 11 was undertaken as a model system here and purified ... -
Repressor of temperate mycobacteriophage LI harbors a stable C-terminal domain and binds to different asymmetric operator DNAs with variable affinity (M-T-2007)
(BIOMED CENTRAL LTD, 2007-06-28)Background: Lysogenic mode of life cycle of a temperate bacteriophage is generally maintained by a protein called 'repressor'. Repressor proteins of temperate lambdoid phages bind to a few symmetric operator DNAs in order ... -
Probing the antiprotease activity of lambda CIII, an inhibitor of the Escherichia coli metalloprotease HflB (FtsH)
(AMER SOC MICROBIOLOGY, 2007-11)The CIII protein encoded by the temperate coliphage lambda acts as an inhibitor of the ubiquitous Escherichia coli metalloprotease HflB (FtsH). This inhibition results in the stabilization of transcription factor lambda ... -
Three-dimensional models of NB-ARC domains of disease resistance proteins in tomato, arabidopsis, and flax
(ADENINE PRESS, 2008-02)Three dimensional models of NB-ARC domains in five different proteins were constructed based on the recently published crystal structure of the apoptotic protease activating factor 1, of which two are for tomato species, ... -
Bipolar spindle frequency and genome content are inversely regulated by the activity of two N-type kinesins in Entamoeba histolytica
(WILEY-BLACKWELL, 2008-06)Bipolar microtubular spindles are seen infrequently in Entamoeba histolytica trophozoites while monopolar or radial microtubular assemblies are common. Additionally, heterogeneity in nuclear DNA content and multi-nucleation ... -
Entamoeba histolytica encodes unique formins, a subset of which regulates DNA content and cell division
(AMER SOC MICROBIOLOGY, 2008-06)The formin family of proteins mediates dynamic changes in actin assembly in eukaryotes, and therefore it is important to understand the function of these proteins in Entamoeba histolytica, where actin forms the major ... -
Genome re-duplication and irregular segregation occur during the cell cycle of Entamoeba histolytica
(SPRINGER/PLENUM PUBLISHERS, 2007-12)Heterogeneity of genome content is commonly observed in axenic cultures of Entamoeba histolytica. Cells with multiple nuclei and nuclei with heterogenous genome contents suggest that regulatory mechanisms that ensure ... -
Interaction geometry involving planar groups in protein-protein interfaces
(WILEY-BLACKWELL, 2007-04)The geometry of interactions of planar residues is nonrandom in protein tertiary structures and gives rise to conventional, as well as nonconventional. (X-H center dot center dot center dot pi, X-H center dot center dot ... -
Peptide segments in protein-protein interfaces
(INDIAN ACADEMY SCIENCES, 2007-01)An important component of functional genomics involves the understanding of protein association. The interfaces resulting from protein-protein interactions - (i) specific, as represented by the homodimeric quaternary ... -
Macromolecular recognition in the Protein Data Bank
(WILEY-BLACKWELL, 2007-01)Crystal structures deposited in the Protein Data Bank illustrate the diversity of biological macromolecular recognition: transient interactions in protein-protein and protein DNA complexes and permanent assemblies in ... -
Geometry of nonbonded interactions involving planar groups in proteins
(PERGAMON-ELSEVIER SCIENCE LTD, 2007-09)Although hydrophobic interaction is the main contributing factor to the stability of the protein fold, the specificity of the folding process depends on many directional interactions. An analysis has been carried out on ... -
Secondary structure based analysis and classification of biological interfaces: identification of binding motifs in protein-protein interactions
(OXFORD UNIV PRESS, 2007-08-01)Motivation: The increasing amount of data on protein-protein interaction needs to be rationalized for deriving guidelines for the alteration or design of an interface between two proteins. Results: We present a detailed ... -
Immobilization of long-lived radionuclides Eu-152,Eu-154 by selective bioaccumulation in Saccharomyces cerevisiae from a synthetic mixture of Eu-152,Eu-154, Cs-137 and Co-60
(ELSEVIER SCIENCE SA, 2008-06-01)Yeast cells of Saccharomyces cerevisiae were found to accumulate Eu-152.154 radioisotopes selectively from a synthetic mixture of Eu-152.154, Cs-137, and Co-60 radiotracers at neutral pH in both trace and macro level. The ... -
Anti-mitotic activity of colchicine and the structural basis for its interaction with tubulin
(JOHN WILEY & SONS, 2008-01)In this review, an attempt has been made to throw light on the mechanism of action of colchicine and its different analogs as anti-cancer agents. Colchicine interacts with tubulin and perturbs the assembly dynamics of ... -
Deuterium oxide stabilizes conformation of tubulin: A biophysical and biochemical study
(KOREAN SOCIETY BIOCHEMISTRY & MOLECULAR BIOLOGY, 2008-01-31)The present study was aimed to elucidate the mechanism of stabilization of tubulin by deuterium oxide (D2O). Rate of decrease of tryptophan fluorescence during aging of tubulin at 4 degrees C and 37 degrees C was significantly ... -
Overexpression of a delayed early gene hlg1 of temperate mycobacteriophage L1 is lethal to both M-smegmatis and E-coli
(KOREAN SOCIETY BIOCHEMISTRY & MOLECULAR BIOLOGY, 2008-05-31)Two genes of temperate mycobacteriophage L5, namely, gp63 and gp64, were hypothesized to be toxic to M. smegmatis. An identical L5 gp64 ortholog (designated hlg1) was cloned from homoimmune mycobacteriophage L1 and ... -
Digestion of the lambda cI Repressor with Various Serine Proteases and Correlation with Its Three Dimensional Structure
(ADENINE PRESS, 2008-12)Partial proteolysis of the lambda cl repressor has been carried Out systematically with trypsin. chymortrypsin, elastase, endoproteinase Glu-C, kallikrein, and thrombin. The cleavage sites have been determined by (i) ... -
Inter-Cellular Variation in DNA Content of Entamoeba histolytica Originates from Temporal and Spatial Uncoupling of Cytokinesis from the Nuclear Cycle
(PUBLIC LIBRARY SCIENCE, 2009-04)Accumulation of multiple copies of the genome in a single nucleus and several nuclei in a single cell has previously been noted in Entamoeba histolytica, contributing to the genetic heterogeneity of this unicellular ... -
Entamoeba Shows Reversible Variation in Ploidy under Different Growth Conditions and between Life Cycle Phases
(PUBLIC LIBRARY SCIENCE, 2008-08)Under axenic growth conditions, trophozoites of Entamoeba histolytica contain heterogenous amounts of DNA due to the presence of both multiple nuclei and different amounts of DNA in individual nuclei. In order to establish ... -
E. coli HflX interacts with 50S ribosomal subunits in presence of nucleotides
(ACADEMIC PRESS INC ELSEVIER SCIENCE, 2009-02-06)HflX is a GTP binding protein of unknown function. Based on the presence of the hflX gene in hflA operon, HflX was believed to be involved in the lytic-lysogenic decision during phage infection in Escherichia coli. We find ...