Department of Biochemistry: Recent submissions
Now showing items 81-100 of 237
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Direct CIII-HflB interaction is responsible for the inhibition of the HflB (FtsH)-mediated proteolysis of Escherichia coli sigma(32) by lambda CIII
(WILEY-BLACKWELL, 2008-10)The CIII protein of bacteriophage lambda exhibits antiproteolytic activity against the ubiquitous metalloprotease HflB (FtsH) of Escherichia coli, thereby stabilizing the lambda CII protein and promoting lysogenic development ... -
The natural naphthoquinone plumbagin exhibits antiproliferative activity and disrupts the microtubule network through tubulin binding
(AMER CHEMICAL SOC, 2008-07-29)Plumbagin (5-hydroxy-2-methyl-1,4-naphthoquinone), a naphthoquinone isolated from the roots of Plumbaginaceae plants, has potential antiproliferative activity against several tumor types. We have examined the effects of ... -
Binding of Indanocine to the Colchicine Site on Tubulin Promotes Fluorescence, and Its Binding Parameters Resemble Those of the Colchicine Analogue AC
(AMER CHEMICAL SOC, 2009-02-24)Indanocine, a synthetic indanone, has shown potential antiproliferative activity against several tumor types. It is different from many other microtubule-disrupting drugs, because it displays toxicity toward multidrug ... -
Cavities and Atomic Packing in Protein Structures and Interfaces
(PUBLIC LIBRARY SCIENCE, 2008-09)A comparative analysis of cavities enclosed in a tertiary structure of proteins and interfaces formed by the interaction of two protein subunits in obligate and non-obligate categories (represented by homodimeric molecules ... -
Protein-protein interaction and quaternary structure
(CAMBRIDGE UNIV PRESS, 2008-05)Protein-protein recognition plays an essential role in structure and function. Specific non-covalent interactions stabilize the structure of macromolecular assemblies, exemplified in this review by oligomeric proteins and ... -
pi-Turns: types, systematics and the context of their occurrence in protein structures
(BIOMED CENTRAL LTD, 2008-09-22)Background: For a proper understanding of protein structure and folding it is important to know if a polypeptide segment adopts a conformation inherent in the sequence or it depends on the context of its flanking secondary ... -
Structural segments and residue propensities in protein-RNA interfaces: comparison with protein-protein and protein-DNA complexes
(Biomedical Informatics, 2008-07-14)The interface of a protein molecule that is involved in binding another protein, DNA or RNA has been characterized in terms of the number of unique secondary structural segments (SSSs), made up of stretches of helix, strand ... -
Inactivation of indispensable bacterial proteins by early proteins of bacteriophages: Implication in antibacterial drug discovery
(BENTHAM SCIENCE PUBL LTD, 2008-06)Bacteriophages utilize host bacterial cellular machineries for their own reproduction and completion of life cycles. The early proteins that phage synthesize immediately after the entry of their genomes into bacterial cells ... -
The delayed early gene G23 of temperate mycobacteriophage L1 regulates the expression of deoxyribonuclease, the product of another delayed early gene of the phage
(POLSKIE TOWARZYSTWO MIKROBIOLOGOW-POLISH SOCIETY OF MICROBIOLOGISTS, 2008)To get clues about the genes as well as the gene regulatory circuit controlling the lytic development of temperate mycobacteriophage L1, previously we screened several conditional lethal mutants of L1 and characterized ... -
Virulence plasmid of Aeromonas hydrophila induces macrophage apoptosis and helps in developing systemic infection in mice
(ACADEMIC PRESS, 2009-02)Pathogenic Aeromonas hydrophila Strain AO1 bears a 21 kb plasmid encoding several virulence determinants. Infection studies revealed that this isolate induced cytotoxicity in BALB/c mice splenic macrophages involving ... -
Moderately thermostable phage Phi 11 Cro repressor has novel DNA-binding capacity and physicochemical properties
(KOREAN SOCIETY BIOCHEMISTRY & MOLECULAR BIOLOGY, 2009-03-31)The temperate Staphylococcus aureus phage Phi 11 harbors cl and cro repressor genes similar to those of lambdoid phages. Using extremely pure Phi 11 Cro (the product of the Phi 11 cro gene) we demonstrated that this protein ... -
Antibiotics, arsenate and H2O2 induce the promoter of Staphylococcus aureus cspC gene more strongly than cold
(WILEY-BLACKWELL, 2009-04)Proteins expressed by the bacterial cold shock genes are highly conserved at sequence level and perform various biological functions in both the cold-stressed and normal cells. To study the effects of various agents on the ... -
Intgeom: A server for the calculation of the interaction geometry between planar groups in proteins
(OMICS Pub., 2009-01)IntGeom is a server for the calculation of the relative orientation between any two planar groups in protein side chains. IntGeom1 considers ten planar groups, while IntGeom2 is meant for studying the contact between a ... -
RecA-Mediated Cleavage of lambda cI Repressor Accepts Repressor Dimers: Probable Role of Prolyl Cis-Trans Isomerization and Catalytic Involvement of H163, K177, and K232 of RecA
(ADENINE PRESS, 2009-10)The λ cI repressor is found to be cleaved in the presence of activated RecA in its DNAbound dimeric form at a rate similar to that in the absence of operator DNA in contrast to previous studies inferring repressor monomer ... -
Genistein Arrests Cell Cycle Progression of A549 Cells at the G2/M Phase and Depolymerizes Interphase Microtubules through Binding to a Unique Site of Tubulin
(AMER CHEMICAL SOC, 2010-03-02)Genistein (40,5,7-trihydroxyisoflavone), an isoflavone, is a major constituent of soyfoods. It has potential antiproliferative activity against several tumor types. We have examined the effect of genistein on cellular ... -
Fluorescence Spectroscopic Methods to Analyze Drug–Tubulin Interactions
(ELSEVIER ACADEMIC PRESS, 2010)Fluorescence spectroscopy has been extensively used to characterize ligand binding to tubulin and microtubules. The inherent advantages of fluorescence spectroscopic methods lie in their ease, sensitivity to local environmental ... -
HflD, an Escherichia coli protein involved in the lambda lysis-lysogeny switch, impairs transcription activation by lambda CII
(ELSEVIER SCIENCE, 2010-01-15)The CII protein of bacteriophage lambda is the key regulator for the lytic–lysogenic choice of the viral lifecycle. An unstable homotetrameric transcription activator of the three phage promoters pE, pI and paQ, λCII is ... -
Specific hydrophobic residues in the alpha 4 helix of lambda CII are crucial for maintaining its tetrameric structure and directing the lysogenic choice
(SOC GENERAL MICROBIOLOGY, 2010-01)The CII protein of the temperate bacteriophage l is the decision-making factor that determines the viral lytic/lysogenic choice. It is a homotetrameric transcription activator that recognizes and binds specific direct ... -
Antagonistic effects Na＋ and Mg2＋ on the structure, function, and stability of mycobacteriophage L1 repressor
(KOREAN SOCIETY BIOCHEMISTRY & MOLECULAR BIOLOGY, 2009-05-31)Temperate mycobacteriophage L1 encodes an unusual repressor (CI) for regulating its lytic-lysogenic switching and, in contrast to the repressors of most temperate phages, it binds to multiple asymmetric operator DNAs. Here, ... -
Properties of HflX, an Enigmatic Protein from Escherichia coli
(AMER SOC MICROBIOLOGY, 2009-04-01)The Escherichia coli gene hflX was first identified as part of the hflA operon, mutations in which led to an increased frequency of lysogenization upon infection of the bacterium by the temperate coliphage lambda. Independent ...